Lab 4 – Protists & Fungi

Lab 4 – Protists & Fungi. Name:

Lab 4 – Protists & Fungi

Objectives

1. Describe the differences between Protists, Fungi and Prokaryotic cells.

2. Describe how to separate Protists based on their nutrition.

3. Look at examples of Protists and label specified structures.

4. Describe how Fungi obtain nutrients.

5. Look at examples of Fungi and label specified structures.

Reading assignment: Read 16.6 – 16.9: There is also a power point of this material.

For this worksheet you will search the internet for examples of the given organism. You must list the reference (web site) that you used for each picture that you copy into the worksheet. You need to choose unlabeled photographs of microscope slides that show all of the structures, that you will then label. Refer to the file “How to Label an Image” found on Bb > Lab Assignments > Lab Background Information.

List 2 differences between prokaryotic bacteria and protists.

A Paramecium caudatum is a unicellular heterotroph. What does this mean?

Phytoplanton are marine autotrophs. What does this mean?

Protists are eukaryotic. Is it possible for a prokaryotic organism to be an autotroph? Explain.

A. Protozoa

1. Amoeba proteus – Using the internet find a photograph of a slide of an amoeba. Copy the photograph (listing the source) into this worksheet and label the nucleus and pseudopod.

What is the pseudopod?

How do these protists ingest food?

2. Paramecium caudatum – Find a photograph of a slide of a paramecium and label the nucleus.

What structure allows this organism to move?

3. Plasmodium vivax is also a protozoan and a parasite.

What disease results when humans are infected with this organism?

How do humans acquire this protozoan?

Please watch the video on Plasmodium’s life cycle:

What cells does the parasite target in the human host?

Where does sexual reproduction take place in the parasite’s life cycle?

B. Algae

1. Volvox (colonial algae) – Find a photograph of a slide of Volvox and label the parent colony, juvenile (or daughter) colony, and vegetative cells.

2. Spirogyra– Find a photograph of a slide of Spirogyra in the asexual stage and label the chloroplasts and cell wall.

Find a second photograph of a slide of Spirogyra in the sexual stage and label the zygotes, female filament (with zygote), male filament (empty) and conjugation tubules.

What is conjugation?

C. Fungi

List 2 differences between protists and fungi.

Describe how fungi obtain nutrients.

List 2 problems caused by fungi and 2 benefits provided by fungi.

1. Yeast (unicellular fungus) – Find a photograph of a slide of yeast.

2. Breadmold – Rhizopus. Find a photograph of a slide of Rhizopus in the asexual stage and label the hyphae, sporangia, and spores.

Find a second photograph of a slide of Rhizopus in the sexual stage and label the gametangium and zygospore.

3. Coprinus Mushroom – Find a photograph of a slide of Coprinus (not a picture of a mature mushroom) and label the cap, stalk, gills, and spores

Once you have completed the worksheet you need to submit it using Blackboard.

Take Lab Quiz 4. This quiz covers the information from sections 16.6 – 16.9.

These are the organisms that you will find images of and the structures you must identify.

Kingdom Protista

Protozoa

Amoeba proteus – pseudopodia, nucleus

Paramecium caudatum – nucleus

Algae

Volvox – multicellular colonial alga

parent colony juvenile colony vegetative cells

Spirogyra – multicellular filamentous alga (2 drawings)

chloroplasts pyrenoids cell walls

female filament zygotes male filament conjugation tubes

Kingdom Fungi

Yeast

Breadmold – Rhizopus (2 drawings)

asexual: hyphae sporangia spores

sexual: gametangium zygospore

Coprinus Mushroom

cap stalk gills spores

Lab 4 – Protists & Fungi

 
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Virtual Lab 5: Ecosystem simulator

Virtual Lab 5: Ecosystem simulator. Virtual Lab 5: Ecosystem simulator

Read the Overview and launch this ecolosystem simulator . Familiarize yourself with the simulator interface. Notice that you can control which species are present in your environment initially and what the diets of each species are. The types of species possible in the program are Plants (A,B,C), Herbivores (A,B,C), Omnivores (A,B) and one top Predator. You can control the diet of each by indicating what they feed on. By setting up different starting configurations you can investigate the evolution of this simulated ecological system.

A. In a couple of sentences describe what happens when you start with only two (A&B) and then all three plant species present.

When only A & B plant species are there and we run the stimulator, the population of Plant a increases and becomes maximum and that of plant B decreases to 0. As the time passes by, species of A decreases and that of B increases such that in long run we have equal distribution of Species A & B.

When we run the simulator with all the three plant species, The consequence of Plant A & B is same but there is no plant of type C.

In this case there are only producers. They harness the sun energy and grow. The favourable ones boom and the others are overshadowed in the process.

B. Describe how many herbivores and omnivores you added (and what they eat) in order to create an ecosystem in which all three plant species can coexist. (if you cannot accomplish the survival of Plant C describe your best configuration. Describe your ecologies by identifying the species present and their diet, for instance:

Omnivore A eats Herbivore A, Herbivore A eats plant A and plant B, Herbivore B eats plant A, All plants present.

All the three varieties of plant A, B & C co-exists when we add Herbivore A, B, & C

Herbivore A eats Plant A & C, Herbivore B eats Plant A & B and Herbivore C Eats Plant B & C.

Omnivore A eats Herbivore A & B and Omnivore B eats Herbivore B & C

Top Predator eats both Omnivore A & B. In this ecosystem all the varieties have a co-existence.

C. If you can accomplish part B, see if you can get all of the species to coexist. (limit your time on this entire experiment to 90 minutes)

D. If we assume that this simulation is a reasonable oversimplification of a typical ecosystems food web what does it tell us about biodiversity and ecology- are they robust or fragile? In general is an ecosystem’s biodiversity preserved as it responds to change?

Virtual Lab 6: Evolution: Sex and the Single Guppy

This simulation follows a set of real life experiments in evolution and natural selection. Familiarize yourself with the interface, guppies, guppy predators, and the experiment. Use an “even mix” of the different guppy color types to start. Run three experiments one with each of the combination of predators. Each experiment should run for five or more generations. Type your solutions in bold face text.

•   State the percentage that each color type makes up in your guppy population both before and after you have let five generations pass. With each experiment state a conclusion that is consistent with your observation.

1. Rivulus only

131 guppies

2. Rivulus and Acara

3. Rivulus, Acara and Cichlids.

•   What two selection pressures are operative?

Virtual Lab 7: Anatomy and Dissections

A. Dissections

1. Earthworm

A. Identify items 1 & 2 on the external dorsal (back side) surface of the worm.

B. Identify items 3, 4, & 5 on the external ventral (belly side) of surface of the worm.

C. Identify item 2 in the image of the worm’s internal morphology w/o the digestive tract.

D. Describe sexual reproduction in worms.

2. Fetal Pig

A. Use the Anatomical References guide. To what region of the body does dorsal, ventral, anterior, and posterior refer to?

B. Investigate the Nervous system. The pig is similar to the human in many ways.

Name four large regions (lobes) of the brain and indicate where they are located and what functions they have in humans.

B. Comparative Hominid Anatomy

•   Compare the the skull casts of a chimp, Australopithicus, Homo erectus, neanderthal, and modern Homo sapiens. Be sure to use the lateral view.

•   Describe features that are common and different between the cranial structure of these creatures. What patterns do you see? •   Describe the basic timeline and sequence of evolution for the creatures listed above. Be explicit.

Virtual Lab 8: Human Impact

A. Water footprints

•   Describe the water crisis. How is it impacting women and children globally? What is happening with the Ogalala (be specific)?

Water crisis refers to lack of access to safe water sources. Inadequate drinking water supply is among the world’s major causes of preventable morbidity and mortality.

Water related health burdens are borne by women and children who may forego schooling and childhood in order to transport water from distant sources. Women and children are also often responsible for the gathering of fuel wood both for cooking and for boiling the polluted water.

The High Plains stretch northward from West Texas to Wyoming and South Dakota, and in natural conditions form a dry grassland. There is less than 16 inches of rain a year near the Rockies and in West Texas, but that increases eastward to 28 inches in central Kansas. The rainfall varies a great deal from year to year, however. The steady gradient of increasing rain to the eastward, but varying yearly rainfall, means that the optimal western limit for growing crops such as corn, rather than grazing cattle, shifts each year. This problem is made worse because there is hardly any water surplus: evaporation levels are very close to precipitation levels. Apart from the constraints on farming, this fact means that there is little recharge of ground water from precipitation: ground water percolates only very slowly eastward in underground aquifers from the areas where Rocky Mountain snowmelt recharges them.

The United States Geological Survey (USGS) began intensive research on the Ogallala in 1978. It found that the Ogallala had discharged perhaps 3 maf/yr into springs and rivers before development: this, then, would be a sustainable yield from the aquifer as it used to be, compared with the 1980 pumping rate of 18 maf/yr.

•   Describe what the water footprint is and how it is estimated.

The water footprint of an individual, community or business is defined as the total volume of freshwater used to produce the goods and services consumed by the individual or community or produced by the business. Water use is measured in water volume consumed (evaporated) and/or polluted per unit of time.

A water footprint can be calculated for any well-defined group of consumers (e.g., an individual, family, village, city, province, state or nation) or producers (e.g., a public organization, private enterprise or economic sector). The water footprint is a geographically explicit indicator, not only showing volumes of water use and pollution, but also the locations. However, the water footprint does not provide information on how the embedded water negatively or positively affects local water resources, ecosystems and livelihoods.

•   Report the two or more estimates of your water footprint (and the calculator(s) that you used).

image1.png

http://www.gracelinks.org/1408/water-footprint-calculator

•   Describe three ways that you (or your society) could reduce your water footprint.

We can use public transport, or pooled vehicles for going to office , we can decrease the use of gasoline

We should not waste potable water. We can store rain water and use for watering our plants.

B. Carbon footprints

•   Describe what has happened to atmospheric CO2 over the last 100,000 years and how this is thought to be impacting the climate.

Over the last 100,000 CO2 content has been increasing due to excessive use of fuel, human population, large scale deforestation

In the distant past (prior to about 10,000 years ago), CO2 levels tended to track the glacial cycles. During warm ‘interglacial’ periods, CO2 levels have been higher. During cool ‘glacial’ periods, CO2 levels have been lower.  This is because the heating or cooling of Earth’s surface can cause changes in greenhouse gas concentrations.

•   Describe what the carbon footprint is and how it is estimated.

Carbon footprint is a shorthand to describe the best estimate that we can get of the full climate change impact of something. That something could be anything – an activity, an item, a lifestyle, a company, a country or even the whole world.

An individual’s, nation’s, or organisation’s carbon footprint can be measured by undertaking a GHG emissions assessment or other calculative activities denoted as  carbon accounting . 

•   Report two or more estimates of your carbon footprint (and the calculator(s) that you used).

image2.png

http://www.carbonfootprintofnations.com/content/calculator_of_carbon_footprint_for_nations/

•   Describe two ways that you (or your society) could reduce your carbon footprint.

One of the most popular ways utilizes trees in a process called Carbon Sequestration.  Trees naturally absorb CO2 and release oxygen back into the environment.  You only need to know how many trees it takes to offset your Carbon Footprint.  Every tree is different and absorb these emissions at different rates depending on type and age. 

The most common way to reduce the carbon footprint of humans is to  Reduce, Reuse, Recycle . In manufacturing this can be done by recycling the packing materials, by selling the obsolete inventory of one industry to the industry who is looking to buy unused items at lesser price to become competetive. Nothing should be disposed off into the soil, all the ferrous materials which are prone to degrade or oxidise with time should be sold as early as possible at reduced price.

•   Describe two ways that you would advocate we adapt society-wide in order to reduce our national carbon footprint. Be explcit.

The national carbon footprints can be reduced by reducing our energy consumption. We should use less of Air conditioning and warming systems.

We should use less fuel and try to save water consumption. We should go in for public transport system and use cycles for transportation to nearby areas.

We should recycle material like newspaper use recycled paper. We should recycle rain water and use it for gardens.

Virtual Lab 5: Ecosystem simulator

 
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Characteristics of our species and the role we play in our ecosystem

Characteristics of our species and the role we play in our ecosystem.

Where do we fit in?

This week you are exploring the characteristics of our species and the role we play in our ecosystem. Two terms commonly used to describe an organism’s place in the environment is their “habitat” and their “niche.” A habitat is defined as an area inhabited by particular species. A niche is defined as the role (job), activities and resources used by an organism. For example, a polar bear’s habitat is the arctic whereas its niche is to swim, eat fish, walruses and seals, and to take care of polar bear cubs. Have you ever considered the characteristics of your habitat and niche or thought about what might happen if you were forced to move and occupy another habitat, and switch to another niche? This Assignment will give you the opportunity to compare and contrast your current habitat and niche with an assigned niche (described below).

Your essay should include:

  • A description of your personal habitat and your assigned habitat highlighting the similarities and differences.
  • A description of your personal niche and of your assigned niche highlighting the similarities and differences.
  • Consider what characteristics would provide a survival advantage to you or to the individuals who currently occupy each niche and habitat.
  • How have humans adapted to the two habitats and niches?
  • What difficulties might you have living in the assigned niche and why?
  • What types of cultural adaptations have evolved from living in your niche? Your assigned niche? What are the benefits and challenges of these cultural adaptations?
  • Your discussion should consider any biological adaptations as well; things such as dealing with food spoilage, insect vector control, food and waterborne illnesses, etc.
  • Consider the organism you have been assigned to study in your assigned niche. Would you classify it as a biotic or an abiotic component of the environment? Explain your reasoning by applying the characteristics of life covered in Chapter One.

Letter of last name: Assigned Niche

A-G

An Ecologist Studying Polar Bear Behavior in the Arctic

H-N

An Ecologist Studying Sloth Behavior in the Tropical Rainforest

O-T

An Ecologist Studying Cheetah Behavior in the Saharan Desert

U-Z

An Ecologist Studying Saimaa Ringed Seal Behavior in the Boreal Forest of Finland

Characteristics of our species and the role we play in our ecosystem

 
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BIO 101 – Enzymes

BIO 101 – Enzymes.

Catalytic Activity of Enzymes

Introduction

Enzyles are biological molecules that catalyze (speed up) chemrcal reactions. You could call enz;anes the “Builders and Do-ers” m the celi: without them, iife could not occur. Every ceI1 makes hundreds of different enzyxes to carry out the reactions necessary for 1ife. Fortunately for the ce1l, enzlmes are not used up when they calaltlze a reaction. but can be used over and over.

The DNA in each ce11 encodes all the information needed to make its many different enz)rmes. Enzlnnes are relatively large molecules of protein. They are produced u,henever tire cel1″senses” a need for that parlicular enzyne; that is, whenever a job needs to be done in the ce ll which only that enzyne can do it.

The molecule (or molecules) on u,hich an enzyne acts is called its substrate. Enzla:nes are said to be very “specific,” meaning that they recognize only one substrate (or a fei,r, closeiy related substrates) and convert it into a specific product. You could say that each enzl.me can do only one tlpe ofjob. Each en4,rne is specrfic because it is folded urto a particular three-dimensional shape. Wrtirur the folds of each enzyle is the active site, the place u,here the substrate fits and where the chemical reaction takes place.

Enzynes work very quickly, often catalyzing thousands of reactions per minute. The rate at which an ervrme works is influenced by many factors including temperature and pH. Enzl.mes have a temperature and pH at which they work best, and if an enzyne is exposed to extremes of heat or pH it won’t rvork at a1ll The interactions that hold the protein in its particular shape become disrupted under these extreme conditions, and the 3-dirnensional structure unfblds. In this case, the enzyxe is said to be denatured. Other important factors that influence enz),rne activity are the concentration of substrate and the concentration of enzyle. Up to a point, the more substrate that is present, the fuster the reaction. Hou,ever, u,hen the substrate concentration is so high that an enzyne is workilg as fast as it can, fui1her increases of substrate concentration will have no effect on the rate of product formation.

Background

The enzyne that you will study in this experiment is called “catalase.” Its job is to break down its substrate hydrogen peroxide (HZOZ,), wirich is a naturaily occuning poison. Vy’ithout catalase, HZOZ

could krll the cell. The reaction calalyzed by catalase is:

2HyO2+2H2O+Oy

The products remaining alter catalase does its job are oxygen gas and water; two very non-poisonous molecules.

CORNELL”. HHMI

wru A,M

O2OO8 CIBT A Study of Catalase – Teacher Section Page 1

 

 

ln the home and hospita| h,vdrogen peroxide is used as an antiseptic to ciean out wounds. Have you et,er noticed that when hydrogen peroxide is su,abbed on a cut it bubbles? This is because enz),rnes ln the cut fiom your body and from infecting bacteria catallze the rapid degradation of h,vdrogen peroxide into water and oxygen. The bubbles are oxygen.

Catalases are very corrrroll. They are found in almost all celis that grorv in oxygeu, inciuding potato tubers. In this experiment, a blender is used to grilrd up a potato in water to release the catalase from the potato cells. The ground-up potato is filtered through cheesecloth to separate potato skin and celI debris from the liquid which contains n’lost of the cell’s enz),rnes, including catalase. To actualiy measure the catalase activify, small disks are dipped rnto the potato cell extract” \&en this enzyrne-contaimng disk is placed in a solution of hydrogen peroxide, the enzyme begins to work. As rhe catalysis occurs, oxygen is produced, and bubbles of the gas become trapped in the fibers of the disk. When there are enough O2

bubbles. they 1ift the fi1ter to the surface. The speed with which the 02 is produced depends both upon hou,’much enz)4xe is present and on the concentration of the irydrogen peroxide. The more enzyme, the fuster the product (O2) is made. SLmilarly, the higher the concentration of the substrate, hydrogen

peroxide, tire faster the product is made. You can see u,hat happens u,hen you vary either the concentration of enzlnne or the concentration of the hydrogen peroxide.

To do this experiment, five of the teams of students will carry out one version of the experiment using low, medium, and high enz)rne concentration and a constant 1evel of substtate, 1.0%H2a2. Then, the other six teams will do another version of the experiment using low, medium, and high H2O2 concentration u,ith

cell extract diluted to 600/o of its original concentration. At the end of the 1ab, experimental results will be pooled and the class as a u,hole will generate data showing the relationship befiveen catalase activity and both enzl,rne and substrate concentrations.

A third experiment dealing with the effect of pH is provided. This porlion could be performed easily by one or nvo teams of students. It aiso lends itsclf nicely to a teacher demonstration for the entire class.

For an experiment to be meaningful, there must be controls. Three controls imporlant to this lab rviil be demonstrated by your teacirer:

Control #1: A paper disc that has not had potato extract added to it is dipped in H2O2.

Control #2: A paper disc that has been dipped in potato extract is placed in a beaker of water.

Control #3: A paper disc that has first been dipped in boiled potato extract and then placed into a beaker of H2O2.

Observe and record on page 8 what occurs as each control experiment is dernonstrated.

Why is each control important?

What does each control experiment show you?

O2OO8 CIBT Catalytic Activity of Enzymes * Student Section Page2

 

 

N{aterials

Your tearn u,ill need the following suppiies:

. potaio extract (prepared by your teacher)

. 1000 mi flask rvitir distilled/deionized/dechlorinated water o I – 250 ml beaker for potato extract . 200 ml o/o HZOZ solution for the first parl of the experiment

. 3okT12A2 solution to dilute for second part of the experimerrt

. 100 ml graduated cyiinder r g – 100 m1 beakers

. forceps . 40 filter paper disks

. paper towels . stopwatch, if available

. 6A0 catalase . calculator

Experimental Procedure for Teams Working $,ith Various Catalase Concentrations

1. Making the potato extract:

. Watch vour teacher prepare the potato extract as {bllows:

. Cut clean potatoes into chunks (allow one potato per team of students)

. Place the potato chunks in tire blender and add 200 ml of buffer per potato.

. Puree in the blender.

‘ Pour potato puree through four layers of cheesecloth placed in funnel. Collect as much fluid as possible. This fluid contains the enzl.Tne catalase. among many other things that were stored inside the cells of the potato.

‘ Add enough distilled water to bring the final volume to 200 n.rl per potato. Swirl the flask to mix the solution. This willbe arbitrarily designated as “100%” catalase extract. (Each team should have a 2-50 mi beaker containing 200 m1 of 100% catalase.)

2. Together wrth your pafiners, prepare your enz),rne concentrations in the beakers.

. Labei the beakers with tape and pen: 20oh, 40oA, 600 , 80o , and 100%.

‘ Make the appropriate dilutions. For example, if you are doing test #1(20%), measure 8.0 mi of the potato extract using the graduated cylinder and pour into the beaker.

. Rinse the graduated cylinder, then add 32.0 m1 of distilled H2O and stir well with the stirring rod.

O2OO8 CIBT Catalytic Activity of Enzymes – Student Section Page 3

 

 

. Make the rest of the enzyme solutions using the chart beiow as a guide.

Test Extract Concentration Volume of En4mre Volume of Water

#1 20% 8ml 32ml

11^l 40% 16 ml 24 nl

ItJ 60% 24 ml 16 ml

!n t++ 8A% 32ml 8ml

ra0% 40 ml 0ml

. Obtain the flask of 1o/o h,vdrogen peroxide if it is not at your tabie. This is tire substrate for this part of the lab.

3, Now you are ready to begin measurilg the effects of enzyrne concentration on enzyme activity.

. Pour 30 ml of the 1% HZOZ solution into a clean beaker, iabeled “reaction beaker.”

. Pick up a paper disk with a clean forceps. Using the forceps, dunk the disk in your en44ne extract for 5 seconds, until the disk is uniformly moistened but not beaded with shiny drops of liquid”

. Drain it on a piece of paper towei for 5 seconds to remove oxcess enz).rne frorn the disk.

4. The reaction is now ready lo be starled and timed.

. Using forceps. place the filter disk (containing the enzynes) onto the bottom of the “reaction beaker” contauing 1% hydrogen peroxide.

. One person should watch the cloclc/stopwatch, another watch the rising disk. Stop timing as soon as disks have completely ‘lifted off the bottom of the reaction beaker.

. Watch the filter disk. You should see tinybubbles of oxlgen being released as the hydrogen peroxide is broken into water and oxygen by the catalase.

. Record the time in seconds for each trial on the char1. Be precise in your timing and recording.

. Remove the disk and discard rt.

5. Obtain another disk and repeat steps 3-4 exactly as done above.

6. Repeat the experiment a thil’d tilne exactly as above: now you have “triplicate” measurements of the rate of oxygen productio n in lok HZOZ at each particular enzyxe concentration. Average these 3

values and record in the charl below. Repeat this procedure for all the concentrations of enzyne.

O2OO8 CIBT Catalytic Activity of Enzymes – Student Section Page 4

 

 

Data Table / Enzyme Concentration

Test Number

Triall Trial2 Trial 3 Team Average

Class Average

1

20% 7 ,39/’ -?jh /’ ,””,.,,,… /a^n/>’ cn1-r/L ) 2

40% J/tL t L,//y, -)

(L- L./,/

4< /qF

L. i|b I 1 J

60% .7 s/bb , .”$L/qs ,.u? 1) > 1

80% t , (/za I’i-Qt,,/:- I . t–7 *?1 5

100% .0q I /1 | . , r”/, i,2 77)

1. Clean up all materials!

Experimental Procedure for Teams Working with Various Hydrogen Peroxide Concentrations

I . Label the clean beakers with the percent h,vdrogen peroxide that will be used in this part of the experiment: 20 , 1.5o/o,1.Ao ,0.80 ,0.60 and 0.30h. Together with i,our partner, prepare your particular substrate concentrations il each of tire labeled beakers. For example, for test #1, measure 20.0 m1 of the concentrated HZaZ using the graduated c,vhnder and pour urto the beaker. Add 10 ml

of distilied u,ater to the -sraduated

cyiinder and pour into the hydrogen peroxide. Stir well with the strn-urg rod. Rinse the graduated cyhnder. See chart for proportions of hydrogen peroxide and water to mix for each dilution. Place all of the beakers on the table in front of you in order fiom lowest to highest concentration of hydrogen peroxide.

Test # Substrate Concentration Volume ofL120y Volume of Water

1 2.4% 20 ml 10 ml

2 15% 15 ml 15 ml

aJ 1.0% 10 ml 20 ml

4 0.8% 8ml 22ml

5 0.6 % 6ml 24 ml

6 0.3 % 3ml 27 ml

Next, obtain your 60% catalase solution.

Using forceps, dip a disk in the 60% potato extract for 5 seconds, 1et it drain on a paper towel for 5 seconds. Then, using forceps, place the filter (contarning enzynes) on the bottom of the “reaction” beaker Q.A%HZO).

Time how long it takes the disk to rise from the bottom of the beaker to the top of the liquid. Be sure

2.

3a.

b.

CI2008 CIBT Catalytic Activitl’ of Enzymes – Student Section Page 5

 

 

that the disk is placed at the bottom of the irydrogen peroxide before you start to time the experiment.

c. Record the time in seconds in the appropriate space on the chart that fo11ows.

4. Obtain another disc and repeat steps 3a – 3c exactly as before,

5. Repeat the experiment a third time. Now you have triplicate measurements of the rate of oxyger-r production. Average these three values and record on the chart.

6. Repeat this procedure for all of the concentrations ofH2O7.

Data Table / Substrate Concentration

Test Number

Triall Trial2 Triai 3 Team

Average Class

Average

I ggaVo 2 .a’l ,

‘i “/,t, -‘l mnt !L t. I i /qz – 5>fns /*r4 r z 4A% 3,9″,’ 7,’tt /ii, 4,atl?q,I -l Z J , 3

69t7, ” ‘a ‘7

( rr’.’ 1,teft=; ( 7, i^tat) tL i lL t ^r? qs ),/ t 4 80% b,t 7 b , ,:o7 $,1o ly7 * 5,7o! ”, ya Itl s 5

IOO%.S.5 ‘/ 1,zc I d,?$f :ts $,<t%f tuq ]11 ) ,?/. $ , ‘i.*l’*.ae 4 no /(ilairiv ^ ,l I J|u t1. Ciean up all materials! I

O2OO8 CIBT Catalytic Activity of Enzymes – Student Section Page 6

 

 

After the Experiment

Controls

A. &hat is the function of a control?

For control #1, a fiter paper saturated with water rather tiran potato extract was placed tn a beaker of I%H7O2. Horv long does it take for the filter to lift off.r Explairi the significance of the result:

For control #2, a piece of filter paper was saturated with potato extract and then placed in distilied

water. How longdid it take for the filter to lift off.r Explain the significance of the results:

For control #3, 100% catalase was boiled. A filter paper was then saturated with this extract. The disk containhg the extract rvas then piaced in a beaker of 1o/oHZOZ. How long did jt take for the

filter to lifl off? Explain significance of the resuits:

B I . pool your results with those of the rest of the class, record belorv and fi1l in the class average portion of the table for your experiment and also for the other experiment.

O2OO8 CIBT Catalytic Activity of Enzymes – Student Section PageT

 

 

test team 1 teamZ team 3 team 4 team 5 team 6 team 7 team 8

1:20oh >L+ 5 L-)

2:400/o Llt t r (o/1 -LJ (

3:60% Tqt aq 4:80oh

O4/i C

A< (

5:100% ?a 1 7Zt 4/- (-

Data Table for Enzyme Concentration (mean for each test)

2.

Data Table for Substrate Coneentration (mean for each test)

test team I team2 team 3 leam 4 team 5 teasa'{ t?j.fn I ftffi 1:2.0% )(lclt-f ,TF

2: 1.50k 6r -) -n/ () c I Q0, 3: l.0o/o ‘bt

*L'{ . ( tn

4:0.8o/o r{* ?”4*i l?>

5: 0.60/o z{1)) 4t s 7t6 6: 0.3o/o

l-) lL- 9 3zs Lfzs

Plot both the team and the class averages on graph paper. The frst graphs should be “concentration of enz),rne vs. time of reaction.” The x-axis is designated concentration, starting at point 0. The y- axis is 1/t, so you have to do the math on this before you graph your data. The second graph should

be “concentration of substrate vs. time of reaction.” The x-axis is designated concenkation, starting at point 0. The y-axis is again 1/t, so do the math first.

O2OO8 CIBT Catalytic Activity of Enzymes – Student Section Page 8

 

 

-?. Discuss three factors that influence the raie of enzFne action:

4. \4ry did you do the experiment in tripiicate?

02008 CIBT catalytic Activity of Enrymes – student section Page 9

BIO 101 – Enzymes

 
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