Research Method Questions

Research Method Questions. 1. What is the independent variable?

 

 

2. What is the dependent variable?

 

 

3. Which one of the research designs below enables use to determine cause and effect?

 

A. correlational B. descriptive C. experimental D. A & C

 

4. The “scientific method” refers to

A. historical, social-cultural, and ethical contexts in which science takes place. B. the specific procedures, measurements, and instruments used by psychologists to conduct research. C. an abstract concept that describes the logic and methods used to answer questions. D. the development of psychophysical methods for measuring thought processes.

 

5. In a study that investigates the effects of two different doses of a drug on memory performance, memory

performance represents the ____ variable and doses of the drug represent the ____variable.

 

A. correlational; confounding B. experimental; control C. dependent; independent D. independent; dependent

6. When the three requirements for causal inference are met, an experiment is said to be

A. balanced. B. internally valid. C. an independent groups design. D. held constant.

 

 

7. The extent to which findings from a study can be used to describe different populations, settings, and conditions is referred to as

A. sampling. B. internal validity. C. external validity. D. the multimethod approach.

 

8. The statement of a research hypothesis includes

A. an explanation for why a particular behavior is under investigation. B. a predicted outcome and an explanation for the outcome. C. an overview of previous research findings regarding the topic. D. all of these

 

9. You are investigating how sleep affects memory. You assign half of your participants to sleep for 8 hours and half to sleep for 4 hours. You then have participants complete a questionnaire asking them to recall how many times their parents brought them to the zoo as a child. Which of the following statements is NOT true concerning this experiment?

 

A. The independent variable is recall.

B. The dependent variable is recall.

C. The independent variable is amount of sleep.

D. Sleep is expected to affect memory.

 

10. What does an Institutional Review Board (IRB) do?

 

A. Makes sure a proposed study is ethical

B. Makes sure a study was conducted according to the plan

C. Makes sure a study will employ a method that produces quality data

D. A & C

 

11. A researcher computes an inferential statistic to test the difference between mean scores for an experimental group and a control group. The probability of the obtained statistical value for the t-test is .025, which is less than the alpha level of significance (p < .05). The researcher should

A. reduce the alpha level of significance to .025 to form a definite conclusion. B. accept the null hypothesis of no difference between the experimental and control groups because the probability is so small; thus, the independent variable had no effect. C. reject the null hypothesis of no difference between the experimental and control groups because the probability is so small; thus, the independent variable had a reliable effect on the dependent variable. D. neither accept nor reject the null hypothesis of no difference because the probability value of .025 is significantly different than .05.

 

 

12. When participants begin an experiment but fail to complete it, the internal validity of the experiment can be threatened. Which of the following types of subject loss poses the most serious threat to internal validity?

 

A. the loss occurs because of an error by the experimenter. B. the loss leads to different numbers of participants in the groups in the experiment. C. the loss occurs because of equipment failure. D. the loss occurs because of some characteristic of the participant that is related to the outcome of the study.

13. A potential problem that arises when evaluating research findings presented in the media (e.g., television,

magazines, online) is that

A. those reporting the findings have little financial stake in the public’s acceptance of the findings. B. important aspects of the research method are likely not reported. C. the findings may be from research studies conducted in other countries. D. all of these

 

14. A researcher randomly assigns one classroom to a new teaching method and a second classroom to the

control condition (the regular teaching method). This researcher faces the potential problem of confounding due to

A. experimenter effects. B. selective subject loss. C. intact groups. D. extraneous variables.

15. (4 points) You are interested in studying the role that gender plays in math performance and verbal skills.

You study girls and boys who range in age from 6 to 10 years. Write a hypothesis for your experiment.

 

16. Informed consent forms are required for studies to

A. have a contract between subjects and researchers.

B. make subjects aware of what they are signing up for.

C. Protect participants, researchers, and their institutions.

D. All of the above

17. Which of the following is an example of an individual differences (subject) variable?

A. individuals randomly assigned to one of three different treatments in a diabetes study B. an independent variable involving three levels of nicotine in a smoking cessation program C. a law enforcement training program compared over a 4-week or an 8-week period D. individuals classified as introverted or extraverted in a study of alcohol use

 

 

18. Which of the following represents the basic question addressed by the risk/benefit ratio?

A. Are the risks in a study greater than minimal risk? B. Will the researcher’s reputation be enhanced or hurt by the study’s findings? C. Are the benefits (to individual participants and society) of a study greater than the risks? D. Are the benefits gained by individuals greater than the risks posed to society?

 

19. When the probability and magnitude of harm or discomfort anticipated in the research are not greater than

those encountered in daily life or during the performance of routine tests, we say that the risk is

A. minor risk. B. daily risk. C. static risk. D. minimal risk.

 

20. A teacher tried a new method of teaching math to her third-grade students. She compared the scores

on their math test to the scores for students she taught last year using the old teaching method. What are the independent and dependent variables in her study?

A. IV = new teaching method; DV = old teaching method

B. IV = math scores; DV = new teaching method

C. IV = teaching method; DV = math scores

D. IV = math scores; DV = teaching method

21. Explain the difference between deception by omission and deception by commission (you can use examples

to help you explain the difference).

 

 

 

 

22. Match the major approaches to research for each of the following studies.

 

a) descriptive b) relational c) experimental

 

__ Observing that with greater consumption of coffee comes greater clarity of thought

__ Observing how many people do or do not drink coffee and how many people think clearly or poorly

__ Giving half of the people at work coffee, and the other half none, and then comparing their ability to think clearly

 

23. A researcher studying interpersonal communication measured the time that couples made eye contact during a conversation. Which descriptive statistics would most commonly be used to describe the researcher’s duration measurements?

A. mean and standard deviation B. median and difference scores C. mode and correlation D. proportion and percentage

24. The primary means scientists use to establish control in their observations is to

A. systematically manipulate an independent variable in an experiment. B. train multiple observers. C. establish the accuracy and precision of their measurements. D. observe many dependent variables.

 

25. In a study examining the number of proofreading errors make when students are tested while reading under

bright or dim lighting, the number of proofreading errors represents the

A. control variable. B. intervening variable. C. dependent variable. D. independent variable.

 

26. Researchers may not be required to obtain informed consent in which of the following situations?

 

A. when doing research with children B. when doing research that involves more than minimal risk C. when observing behavior in public settings with no intervention D. when asking participants to complete questionnaires on the Internet

 

27. A researcher read a research report indicating that a certain medication was found to be effective when tested on men. The researcher plans to do an experiment testing the effectiveness of the same medication, but in his experiment both men and women will be tested. The researcher is planning to do a

A. partial replication of the experiment to test the reliability and external validity of the finding from the original experiment. B. replication of the experiment to test the internal validity of the original experiment. C. replication of the experiment to test the sensitivity of the original experiment. D. partial replication of the experiment to test the statistical power of the original experiment.

28. The term scientists use to refer to a psychological concept is

 

A. construct. B. validity. C. variability. D. operational definition.

 

29. Which of the following is not required of researchers in an informed consent procedure?

A. description of the nature of the research B. reasons for why deception is used in the research C. information that might influence participants’ willingness to participate D. all of these are required

 

30. If a psychology student wants to do research involving human participants, which of the following has the

ultimate authority to approve, disapprove, or require modifications prior to the approval of the student’s research?

A. the department chairperson B. the university’s Institutional Review Board (IRB) C. the university’s IACUC D. the faculty sponsor of the research

 

31. A researcher develops a brief questionnaire measure of people’s level of optimism. To determine whether

his measure is a good one, he asks a sample of people to complete his questionnaire twice, separated by one month. At the second session, he also asks his participants to complete another measure of optimism—one that has been an accepted measure of optimism for many years. Using this procedure, the researcher is establishing the

A. independent variable of optimism. B. precision and accuracy of his measure. C. reliability and validity of his measure. D. nomothetic and idiographic features of optimism.

 

32. Research has indicated that amount of undergraduate research activity predicts measures of later success in

the profession of psychology. For this prediction to be possible, undergraduate research activity and later success must be

A. circular. B. qualitative. C. causal. D. correlated.

 

33. For a class research project, students conceal themselves in bathroom stalls in order to observe

conversational behavior of individuals at sinks in the washroom. They count the number of words spoken by women and men in their respective washrooms. The most important ethical issue in this research is

A. debriefing. B. informed consent. C. privacy. D. deception.

 

34. Random assignment to conditions works to balance participants’ individual differences across conditions of the experiment by

A. matching individuals on key variables. B. asking individuals to participate in each condition of the experiment. C. forming groups based on an individual differences variable the researcher selects. D. generating groups of participants that are equivalent, on average.

 

35. Identify two ways in which debriefing benefits the participant and one way in which it benefits

the researcher.

 

 

36. (4 points) The procedure for using block randomization in an independent-groups experiment with three conditions (A, B, C) and 60 subjects is to

A. generate twenty random orders of the conditions (e.g., ACB, BAC) and assign subjects one block at a time (i.e., 3 subjects in the first block, 3 in the second block, and so on). B. generate one random order of conditions (e.g., ACB) and test the first 20 subjects in condition A, the second 20 subjects in condition C, and the last 20 subjects in condition B. C. select intact groups of 20 subjects each and randomly assign the groups to condition A, B, or C. D. generate sixty random orders of the conditions, one for each subject.

 

37. When scientists conduct research, they seek to

A. use only real-world settings. B. use only laboratory settings. C. describe the findings only for people and circumstances who participated in their study. D. generalize their findings beyond the people and circumstances used in their study.

 

38. Greater risk in a research study is acceptable when

A. clear and immediate benefits to the participants are expected. B. the research has obvious scientific and social value. C. valid and interpretable results will be produced. D. all of these

 

39. A researcher conducted an experiment in which participants played either a violent or a nonviolent video game. After the game, the researcher measured hostile cognitions. The effect size, Cohen’s d, for the difference in mean hostile cognition between the violent and nonviolent conditions was .83. Based on this, it is possible to state that the video game independent variable had a _______ effect on hostile cognition in this experiment.

A. nonsignificant B. small C. medium D. large

 

40. A(n) __________ is a logically organized set of propositions that serves to define events, describe

relationships among events, and explain the occurrence of events.

A. theory B. intervening variable C. hypothesis D. causal inference

 

 

41. (6 points) A researcher was interested in whether divorce and remarriage influence the extent to which children are sociable. At a nearby school, the researcher classified 5th-graders into one of three groups: intact parents (no divorce), divorced (single-parent families), and remarried parents. The researcher interviewed the 5th-graders and rated their sociability, and also asked them to complete a questionnaire that assessed their level of comfort in different social situations. What type of experimental design did this researcher use?

 

What is the independent variable?

 

What is the dependent variable?

 

42. (8 points) A researcher tested whether exposure to images of very thin fashion models causes young women to be dissatisfied with their own body, compared to exposure to athletic body images or neutral (non-body) images. She randomly assigned 120 women from an introductory psychology course to one of three exposure conditions: very thin female images, athletic female images, or neutral images (e.g., household objects). Each condition had 10 images, projected individually on a large screen. The young women participated in small groups. Each image was displayed for 1 minute, for a total of 10 minutes of exposure. After viewing each image, participants wrote for 30 seconds a description of the image (the participants were led to believe their memory was being tested). After viewing the images, the women completed a questionnaire about satisfaction with their body. Negative scores indicate body dissatisfaction and positive scores indicate satisfaction with their body. The mean scores for each condition were as follows:  

What type of experimental design did this researcher use?

 

Describe the independent variable in this study.

 

Describe the dependent variable in this study.

 

Someone unfamiliar with research methods criticizes the findings by arguing that women who viewed the very thin images probably were more dissatisfied with their bodies even before participating in the experiment. State whether you agree or disagree with this argument and explain your reasoning.

Research Method Questions

 
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M3 Writing Analysis-Biology

M3 Writing Analysis-Biology.

 PLEASE READ THE INSTRUCTION THOROUGHLY:

TEXBOOK CHAPTER: https://online.vitalsource.com/#/books/9780134814216/cfi/155!/4/2@100:0.00

IMPORTANT: Additional resources are welcomed for more support, but the grade associated with the information from the book designated for this class will be the measurement tool to assess your paper. Please review the “plagiarism” screencast below. This will assist with plagiarism flags from your writing assignments. Turnitin is a plagiarism tool used to detect word for word verbiage online.

ASSIGNMENT:

You are a doctor in a hospital, and a patient is experiencing trouble with her skin repairing itself from a cut. The patient is also expecting a child, but the cells in the reproduction development are experiencing malfunction in cell division.

  • Describe the stages of each type of cell reproduction process from a normal patient whose body cells can repair themselves and normal cell division during the reproductive development of the unborn baby.
  • Explain the disadvantages and advantages of each type of cell division.
  • Discuss how the patient experiencing problems with the cells repairing from the cut and the child’s reproduction development malfunctions can alter haploid and diploid cell development.

Length/Formatting Instructions

Length 1000 Words

Font 12 point , Calibri Font, no more than 1″ margins

Program/File Type Submit in Word

Attachments Should be pasted into the Word document if possible

Referencing system APA referencing system is necessary in assignments, especially material copied from the Internet.

M3 Writing Analysis-Biology

 
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Fatty Acids, Nutrition and Health Exercise

Fatty Acids, Nutrition and Health Exercise. Scenario

 

Fatty Acids, Nutrition and Health

 

Students please note : It is assumed that you have an understanding of organic molecules as presented in your text book. If not, you should read chapter 3 in your text book dealing with organic molecules. At a minimum, you need to look over the sections associated with lipids. At the end of the scenario, there are some links listed to web sites that you might find useful.

 

 

Its time for your annual physical examination…

 

Suppose that you were visiting your doctor for an annual physical examination. While you were in the waiting room, you picked up and began to read a pamphlet about dietary fats (lipids) and their impacts on health. In order to remember them later, you typed some of the important points into a file on your laptop. To refresh your memory, and for your convenience (and the rest of the class), these notes can be seen below.

 

Notes on Lipids and Health

 

True lipids (triglycerides)

· composed of a glycerol molecule covalently bound to three fatty acid side chains

· hydrophobic molecules, meaning they are non-polar and don’t mix with water

· a group of triglycerides is called a fat

 

Saturated fats

· solid at room temperature (think of fat on an uncooked steak)

· hydrocarbon chains in fatty acids have no carbon-carbon double covalent bonds

· maximum number of hydrogens are covalently bound to the carbons, thereby “saturating” them

· ( Saturated Fatty Acid )animal fats such as lard and butter are usually high in saturated fatty acids

 

 

 

 

Unsaturated fats

· liquid (oils) at room temperature

· hydrocarbon chains of the fatty acids have at least one (monounsaturated) or more (polyunsaturated) carbon-carbon double covalent bonds

· double bonds cause bends in the molecules and also leave them “unsaturated” with hydrogens

· unsaturated fats are found in plant oils such as olive or canola oil

( Monounsaturated Fatty Acid )

 

Dietary fatty acids

· fatty acids are a necessary component of a complete diet

· fatty acids are found in foods such as fatty meats, plant oils and dairy products

· certain polyunsaturated fatty acids (called essential fatty acids) cannot be synthesized by the human body and must come from the diet

· one such essential fatty acid is linoleic acid which can be found in foods such as sunflower oil and almonds

· both monounsaturated and polyunsaturated fatty acids can be found in plant oils

 

( Polyunsaturated Fatty Acid )

 

 

Trans fats

· are triglycerides that contain trans fatty acids

· Trans means “across” so…

· a trans fatty acid is an unsaturated one in which the hydrogens attached to adjacent carbons in a carbon-carbon double covalent bond are on opposite sides of the molecule

· produced by the process of adding hydrogens to unsaturated vegetable oils

· hydrogenation decreases the number of carbon-carbon double covalent bonds in the molecules and creates what are known as “hydrogenated” or “partially hydrogenated” vegetable oils

· trans configuration gives the fatty acids chemical properties more similar to saturated fatty acids (such as lack of bends in the molecules) and can also lead to some accumulation of “bad” types of cholesterol when consumed

· trans fats are found in any hydrogenated oils, so margarines and shortening made from vegetable oils are major sources

· since they are made from plant oils, these were once touted as being much healthier than saturated animal fats

 

 

· in cis fatty acids, the hydrogens attached to adjacent carbons in a carbon-carbon double covalent bond are on the same side of the molecule.

· Almost all naturally occurring unsaturated fatty acids are cis isomers

 

 

 

 

So, the doctor says your cholesterol is too high…

 

When you were finally called back to a room, your doctor said that a standard analysis of your blood showed that your total cholesterol level was 250mg/dL (a dL=deciliter, or 1/10 of a liter, 100mL). He/she explained that a total cholesterol level of 240mg/dL or above is considered high and is a risk factor for coronary artery disease (CAD).

 

In continuing conversations with your physician, you learned that as a general rule cholesterol is not always “bad.” In fact, cholesterol is a necessary component of cell membranes and is a vital part of normal metabolic processes, including formation of other steroids. In addition to looking at total cholesterol concentration in your blood, your doctor emphasized that it is important to take notice of high density lipoprotein (HDL) cholesterol and low density lipoprotein (LDL) cholesterol, which make up major fractions of the total cholesterol and may be more accurate indicators of CAD risk.

 

Your LDL fraction was found to be 195mg/dL which is considered very high.

 

After seeing your very high LDL number, your physician went on to say that the role of LDLs is to carry cholesterol around in the bloodstream and deposit it where it is needed. Unfortunately, if the level of LDLs becomes too high they can also deposit the cholesterol in arteries, forming “plaques” and clogging the arteries. On the other hand, HDLs are responsible for carrying excess cholesterol away from the arteries to the liver where it can be metabolized or “reprocessed” for other purposes. So, with this in mind, cholesterol bound in LDLs is often called “bad cholesterol” and cholesterol bound in HDLs is called “good cholesterol”. In order to avoid CAD, the average person should attempt to raise the level of HDLs in their blood and lower the level of LDLs, although both are needed at some level in the blood.

 

At this point, your doctor strongly urged you to exercise regularly and watch your diet because consumption of fats can have a profound effect on cholesterol levels in the body. However, it turns out that it is probably the types of fat, not the total amount of fat that you eat that may determine incidence of CAD. In fact, a 2001 study by Frank Hu and colleagues at the Harvard School of Public Health showed that different parts of the world with similar total fat intake had very different amounts of death from CAD. Individuals in countries where the fats eaten were mainly in the form of saturated fats and trans fats had much higher incidences of death from CAD than individuals in countries where the fats were mainly consumed in the form of polyunsaturated fats containing omega-3 fatty acids. In general, the study showed that consumption of saturated fats and trans fats tended to increase LDL cholesterol while consumption of polyunsaturated fats tended to both decrease LDL cholesterol and increase HDL cholesterol.

 

References

Hu, FB, Manson, JE, and Willett, WC. 2001. Types of Dietary Fat and Risk of Coronary Heart Disease: A Critical Review. Journal of the American College of Nutrition 20:5-19.

 

Useful Links:

More explanation about trans fats from the FDA

http://www.fda.gov/Food/LabelingNutrition/ConsumerInformation/ucm109832.htm#unhide

 

Understanding cholesterol numbers website

http://www.webmd.com/cholesterol-management/guide/understanding-numbers

 

Government information about reading nutrition labels

http://www.fda.gov/Food/LabelingNutrition/ConsumerInformation/ucm078889.htm

 

 

Pre-Activity Assignment: Fatty Acids, Nutrition and Health ________________________ Name

Read the Fatty Acids, Nutrition and Health reading and Chapter 3 in the text book and then answer the following questions before class.

 

1. A trans fatty acid is one

a. that has no carbon-carbon double bonds directly adjacent to each other.

b. that is a major component of phospholipids in cell membranes.

c. in which the hydrogens attached to adjacent carbons in a carbon-carbon double covalent bond are on opposite sides of the molecule.

d. in which the hydrogens attached to adjacent carbons in a carbon-carbon double covalent bond are on the same side of the molecule.

e. that is saturated with hydrogens.

 

2. Types of polyunsaturated fatty acids that are necessary in the human diet because they cannot be synthesized by the body are called _____________ fatty acids.

a. essential

b. important

c. trans

d. omega-3

e. hydrophobic

 

3. Generations of Americans were introduced to trans fats in their diet in the form of ____________ which was hailed as a healthy alternative to the saturated fats found in butter and lard.

a. Coconut oil

b. Olive oil

c. Margarine

d. Canola oil

e. Beef tallow

 

4. HDL stands for

a. Highly dense lipid.

b. Hydrogenated dark lipid.

c. High density lipid.

d. Hydrogenated dense lipoprotein.

e. High density lipoprotein.

 

5. In the next class meeting you will work in small collaborative groups to answer four question sets. Each person in the group will act as the ‘facilitator’ for one question set, leading the group discussion, promoting input from each of the other students (who will be acting as ‘discussants’) and formalizing the group response. In the role of a discussant, students provide their knowledge, experience and perspectives, compare and contrast the inputs of other members of the group and collaborate in the formulation of the group response. At the end of the activity, you may be called on to present your group’s answers to one of the question sets (not necessarily the one you were the facilitator for). You will act as both a facilitator and a discussant in the activity.

Make sure you have critically analyzed the Fatty Acids, Nutrition and Health scenario and chapter 3 in the text book so that you are prepared to participate in all aspects of the group activity. Bring the scenario with you to the next class meeting.

 

Activity—Fatty Acids, Nutrition and Health (Critical Question Sets)

1. Plaque Formation in Arteries: High levels of LDL cholesterol in the bloodstream can lead to formation of “plaques” in the arteries.

 

_________________________________

Facilitator

 

 

· What are the potential health consequences of coronary arteries being clogged by “plaques”?

 

 

 

 

 

· Name some specific foods that might lead to increased LDL cholesterol in the bloodstream.

 

 

 

 

 

· What do these foods contain that could cause this?

 

 

 

 

2. Nutrition Labels: Analyze the nutrition label below and answer the following questions.

 

______ ___________________________

Facilitator

 

· How much total fat, saturated fat and trans fat does this product contain per serving?

 

 

 

 

· How many grams of total fat would a person consume if he/she ate the whole container?

 

 

· How many calories are from fat per serving?

 

 

 

· Calculate the percentage of the total calories per serving that come from total fat?

 

 

 

 

· Based on a 2,000 calorie diet, what percent of the USDA’s percent daily value for total fat would be consumed per serving? Calculate the percent if the whole container was consumed?

 

 

 

· Based on what you know about trans fats, do you think there are hydrogenated oils in this product? Explain.

 

 

 

3. Hydrogenation of Oils: Answer the following questions about hydrogenation of vegetable oils.

_________ _____________ ___________

Facilitator

 

· What is the reason behind hydrogenating vegetable oils? What effect does the hydrogenation process have on their chemical and physical properties?

 

 

 

 

 

· Draw both a cis isomer and a trans isomer of a polyunsaturated fatty acid. Which would be produced by the hydrogenation process?

 

 

 

 

 

 

 

 

 

4. Cholesterol Profiles: Answer the following question using what you know about cholesterol and the tables to the right that show health categories based on blood levels of different fractions of cholesterol.

 

___________ _____________________

Facilitator

 

 

· Circle the blood cholesterol profile below that you think would be most desirable? What about this profile made you choose it over the others? Explain why you didn’t choose each of the other two.

 

 

 

 

(a) (b) (c)

 

 

 

Post-Activity Assignment: Fatty Acids, Nutrition and Health ________________________

Name

 

6. Which one of the following diagrams represents a trans fatty acid?

 

 

a.

 

 

 

 

 

 

 

 

b.

 

 

 

 

 

 

 

c.

 

 

 

d.

 

 

 

 

 

 

 

7. Which one of the following would be solid at room temperature?

a. Cis fatty acids

b. Corn oil

c. Peanut oil

d. Saturated fats such as lard (pig fat)

e. Unsaturated fats

 

 

 

8. A true lipid is composed of glycerol and three fatty acids. What type of reaction is used to link each of the fatty acids to a glycerol molecule?

a. Dehydration

b. Hydrolysis

c. Dehydrohalogenation

d. Hydrogenation

e. Hydroxylation

 

9. Food companies can tag their products on the nutrition label as having 0g of trans fats if they have <0.5g of trans fat per serving. What could be found in the ingredients list that is probably a better indicator of the presence of trans fats in foods than the trans fat line on the nutrition label?

a. Lard

b. Hydrogenated oils

c. Palm oil

d. Olive oil

e. Almonds

 

10. Consumption of which of the following is most likely to raise your HDL and also lower your LDL levels?

a. Trans fats

b. Saturated fats

c. Lard

d. Polyunsaturated fats

e. Margarine

 

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Lab 5: Meiosis INSTRUCTIONS: And 3425

Lab 5: Meiosis INSTRUCTIONS: And 3425. lab biology questions
BiolLab_5.docx
BiolLab_5.docx

Your Full Name:

102/103
Lab 5: Meiosis
INSTRUCTIONS:

and submit it via the Assignments Folder by the date listed in the Course
Schedule (under Syllabus).

To conduct your laboratory exercises, use the Laboratory Manual located under
Course Content. Read the introduction and the directions for each exercise/experiment
carefully before completing the exercises/experiments and answering the questions.

Save your Lab 5 Answer Sheet in the following format: LastName_Lab5 (e.g.,
Smith_Lab5).

You should submit your document as a Word (.doc or .docx) or Rich Text Format
(.rtf) file for best compatibility.

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Pre-Lab Questions
1. Compare and contrast mitosis and meiosis.

2. What major event occurs during interphase?

Experiment 1: Following Chromosomal DNA Movement
through Meiosis
Data Tables and Post-Lab Assessment
Trial 1 – Meiotic Division Beads Diagram:
Prophase I

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Metaphase I

Anaphase I

Telophase I

Prophase II

Metaphase II

Anaphase II

Telophase I

Cytokinesis

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Trial 2 – Meiotic Division Beads Diagram:
Prophase I

Metaphase I

Anaphase I

Telophase I

Prophase II

Metaphase II

Anaphase II

Telophase I

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Cytokinesis

Post-Lab Questions
1. What is the ploidy of the DNA at the end of meiosis I? What about at the end of meiosis

II?

2. How are meiosis I and meiosis II different?

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3. Why do you use non-sister chromatids to demonstrate crossing over?

4. What combinations of alleles could result from a crossover between BD and bd

chromosomes?

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5. How many chromosomes were present when meiosis I started?

6. How many nuclei are present at the end of meiosis II? How many chromosomes are in

each?

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7. Identify two ways that meiosis contributes to genetic recombination.

8. Why is it necessary to reduce the number of chromosomes in gametes, but not in other

cells?

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9. Blue whales have 44 chromosomes in every cell. Determine how many chromosomes

you would expect to find in the following:

a.i.

Sperm Cell:

a.ii.

Egg Cell:

a.iii.

Daughter Cell from Mitosis:

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a.iv.

Daughter Cell from Meiosis II:

10. Research and find a disease that is caused by chromosomal mutations. When does the

mutation occur? What chromosomes are affected? What are the consequences?

11. Diagram what would happen if sexual reproduction took place for four generations using

diploid (2n) cells.

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Experiment 2: The Importance of Cell Cycle Control
Data Tables and Post-Lab Assessment
1.

2.

3.

4.

5.

Post-Lab Questions
1. Record your hypothesis from Step 1 in the Procedure section here.

2. What do your results indicate about cell cycle control?

3. Suppose a person developed a mutation in a somatic cell which diminishes the

performance of the body’s natural cell cycle control proteins. This mutation resulted in
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cancer, but was effectively treated with a cocktail of cancer-fighting techniques. Is it possible
for this person’s future children to inherit this cancer-causing mutation? Be specific when
you explain why or why not.

4. Why do cells which lack cell cycle control exhibit karyotypes which look physically

different than cells with normal cell cycle.

5. What are HeLa cells? Why are HeLa cells appropriate for this experiment?

Experiment 1:
Following
Chromosomal DNA
Movement through
Meiosis
In this experiment, you
will model the movement
of the chromosomes
through meiosis I and II to
create gametes.

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Materials

2 Sets of Different Colored Pop-it® Beads (32 of e
may be any color)
8 5-Holed Pop-it® Beads (used as centromeres)
Procedure:
Part 1: Modeling Meiosis
without Crossing Over
As prophase I begins, the
replicated chromosomes
coil and condense…
1.

Build a pair of
replicated,
homologous
chromosomes. 10
beads should be
used to create each
individual sister
chromatid (20
beads per
chromosome pair).
Two five-holed
beads represent
each centromere.
To do this…

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Figure 3: Bead set-up. The blue beads
represent one pair of sister chromatids
and the black beads represent a second
pair of sister chromatids. The black and
blue pair are homologous.
a.

Start with
20 beads of
the same
color to
create your
first sister
chromatid
pair. Five
beads must
be snapped
together for
each of the
four
different
strands.
Two
strands
create the
first
chromatid,
and two
strands
create the
second
chromatid
with a 5holed bead
at the
center of
each
chromatid.
This creates
an “I”
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shape.
b.

Connect the
“I” shaped
sister
chromatids
by the 5holed beads
to create
an “X”
shape.

c.

Repeat this
process
using 20
new beads
(of a
different
color) to
create the
second
sister
chromatid
pair.

2.

Assemble a second
pair of replicated
sister chromatids;
this time using 12
beads, instead of
20, per pair (six
beads per each
complete sister
chromatid strand).

3.

Pair up the
homologous
chromosome pairs
created in Step 1
and 2. DO NOT
SIMULATE
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CROSSING
OVER IN THIS
TRIAL. You will
simulate crossing
over in Part 2.
4.

Configure the
chromosomes as
they would appear
in each of the
stages of meiotic
division (prophase
I and II, metaphase
I and II, anaphase I
and II, telophase I
and II, and
cytokinesis).

5.

Diagram the
corresponding
images for each
stage in the
sections titled
“Trial 1 – Meiotic
Division Beads
Diagram”. Be sure
to indicate the
number of
chromosomes
present in each cell
for each phase.

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Figure 4: Second set of replicated
chromosomes.
6.

Disassemble the
beads used in Part
1. You will need to
recycle these beads
for a second
meiosis trial in
Steps 8 – 13.

Part 1 – Meiotic Division
Beads Diagram
Prophase I
Metaphase I
Anaphase I
Telophase I
Prophase II
Metaphase II
Anaphase II

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Telophase II
Cytokinesis
Part 2: Modeling Meiosis
with Crossing Over
7.

Build a pair of
replicated,
homologous
chromosomes. 10
beads should be
used to create each
individual sister
chromatid (20
beads per
chromosome pair).
Two five-holed
beads represent
each centromere.
To do this…
a.

Start with
20 beads of
the same
color to
create your
first sister
chromatid
pair. Five
beads must
be snapped
together for
each of the
four
different
strands.
Two

© eScience Labs, LLC 2014

strands
create the
first
chromatid,
and two
strands
create the
second
chromatid
with a 5holed bead
at the
center of
each
chromatid.
This creates
an “I”
shape.
b.

Connect the
“I” shaped
sister
chromatids
by the 5holed beads
to create
an “X”
shape.

c.

Repeat this
process
using 20
new beads
(of a
different
color) to
create the
second
sister
chromatid
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pair.
8.

Assemble a second
pair of replicated
sister chromatids;
this time using 12
beads, instead of
20, per pair (six
beads per each
complete sister
chromatid strand).
Snap each of the
four pieces into a
new five-holed
bead to complete
the set up.

9.

Pair up the
homologous
chromosomes
created in Step 8
and 9.

10.

SIMULATE
CROSSING
OVER. To do this,
bring the two
homologous pairs
of sister
chromatids
together (creating
the chiasma) and
exchange an equal
number of beads
between the two.
This will result in
chromatids of the
same original
length, there will
now be new

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combinations of
chromatid colors.
11.

Configure the
chromosomes as
they would appear
in each of the
stages of meiotic
division (prophase
I and II, metaphase
I and II, anaphase I
and II, telophase I
and II, and
cytokinesis).

12.

Diagram the
corresponding
images for each
stage in the section
titled “Trial 2 Meiotic Division
Beads Diagram”.
Be sure to indicate
the number of
chromosomes
present in each cell
for each phase.
Also, indicate how
the crossing over
affected the genetic
content in the
gametes from Part1
versus Part 2.

Part 2 – Meiotic Division
Beads Diagram:
Prophase I
Metaphase I

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Anaphase I
Telophase I
Prophase II
Metaphase II
Anaphase II
Telophase II
Cytokinesi

Experiment 2: The Importance of Cell Cycle Control
Some environmental factors can cause genetic mutations which result in a
lack of proper cell cycle control (mitosis). When this happens, the possibility
for uncontrolled cell growth occurs. In some instances, uncontrolled growth
can lead to tumors, which are often associated with cancer, or other biological
diseases.
In this experiment, you will review some of the karyotypic differences which
can be observed when comparing normal, controlled cell growth and
abnormal, uncontrolled cell growth. A karyotype is an image of the complete
set of diploid chromosomes in a single cell.
Procedure
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Materials
*Computer Access
*Internet Access

1.

*You Must Provide

Begin by constructing a hypothesis to explain what differences you
might observe when comparing the karyotypes of human cells which
experience normal cell cycle control versus cancerous cells (which
experience abnormal, or a lack of, cell cycle control). Record your
hypothesis in Post-Lab Question 1.
Note: Be sure to include what you expect to observe, and why you think
you will observe these features. Think about what you know about
cancerous cell growth to help construct this information

2.

Go online to find some images of abnormal karyotypes, and normal
karyotypes. The best results will come from search terms such as
“abnormal karyotype”, “HeLa cells”, “normal karyotype”, “abnormal
chromosomes”, etc. Be sure to use dependable resources which have
been peer-reviewed

3.

Identify at least five abnormalities in the abnormal images. Then, list and
draw each image in the Data section at the end of this experiment. Do
these abnormalities agree with your original hypothesis?
Hint: It may be helpful to count the number of chromosomes, count the
number of pairs, compare the sizes of homologous chromosomes, look
for any missing or additional genetic markers/flags, etc.

Data
1.
2.
3.

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4.
5.
Click here to download and solve a few questions.

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Bio lab 5 answer.docx
Bio lab 5 answer.docx

Your Full Name:

102/103
Lab 5: Meiosis
INSTRUCTIONS:

and submit it via the Assignments Folder by the date listed intheCourse
Schedule (under Syllabus).

To conduct your laboratory exercises,…

Lab 5: Meiosis INSTRUCTIONS: And 3425

 
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