Environmental Contribution

Environmental Contribution. QuestionPart 1 of 1 –
Question 1 of 25
4.0 Points
Which of the following people is INCORRECTLY matched with his/her environmental contribution?
A.Rachel Carson: Silent Spring
B.John Muir: Sierra Club
C.Paul Ehrlich: The Population Bomb
D.Theodore Roosevelt: Civilian Conservation Corps
Part 1 of 1 –
Question 2 of 25
4.0 Points
DDT was sprayed in a Long Island salt marsh over a period of years for mosquito control. A portion of the food web is as follows:
algae -> shrimp-> American eel -> Atlantic needlefish -> ring-billed gull
In which organism would biological magnification be MOST evident?
A.American eel
B.Ring-billed gull
C.Algae
D.Shrimp
Part 1 of 1 –
Question 3 of 25
4.0 Points
Which of the following statements about biogeochemical cycles is true?
A.They only include processes conducted by or within living organisms.
B.They pertain only to the abiotic environment.
C.They describe the movement of water and other materials through an ecosystem.
D.They only pertain to exchanges and interactions that occur within the atmosphere.
Part 1 of 1 –
Question 4 of 25
4.0 Points
Which of the following would be a symptom of acute toxicity?
A.asthma
B.arthritis
C.dizziness
D.cancer
Part 1 of 1 –
Question 5 of 25
4.0 Points
Which of the following series is organized in successive steps according to the levels of organization used by ecologists?
A.population -> ecosystem -> community
B.population -> community -> ecosystem
C.species -> community -> biosphere
D.species -> ecosystem -> population
Part 1 of 1 –
Question 6 of 25
4.0 Points
Corporations and governments began to take NEPA seriously after which of the following events?
A.Further legislation was added to strengthen NEPA.
B.The publication of Rachel Carson’s book Silent Spring.
C.The creation of the EPA.
D.Environmental groups began suing individuals and organizations based on environmental impact statements.
Part 1 of 1 –
Question 7 of 25
4.0 Points
This figure BEST illustrates:

Graphic
A.how our atmosphere maintains its temperature.
B.how energy is recycled in an ecosystem.
C.the one-way flow of energy in an ecosystem.
D.the importance of decomposers in an ecosystem.
Part 1 of 1 –
Question 8 of 25
4.0 Points
The most common method of determining whether a chemical causes cancer is to expose groups of laboratory animals, such as rats, to various large doses and count how many animals develop cancer at the different levels. All of the following are problems with this approach in terms of understanding the health risks to humans EXCEPT:
A.This method is indirect and uncertain as human and rats are different organisms and may respond differently to exposure to the same chemical.
B.For expediting the research process, in lab settings rats are exposed to massive doses of suspected carcinogens relative to body size whereas humans are usually exposed to much lower amounts.
C.For ethical reasons, rats in lab settings are given very small doses of suspected carcinogens relative to body size whereas humans are usually exposed to much higher amounts
D.Risk assessment assumes that we can extrapolate from studies in rats to determine the expected rates of cancer in humans but extrapolating from one species to another and from one dose level to another is uncertain and may overestimate or underestimate a toxicant’s danger.
Part 1 of 1 –
Question 9 of 25
4.0 Points
How might the clearing of roads and pristine areas like the one shown below lead to an increase in infectious disease?
Graphic
A.Cars on the road can carry the diseases to cities.
B.Typhoid and cholera can spread from wild animals to drinking water.
C.The creation of drainage ditches provides habitat for disease carrying mosquitoes.
D.There is an increased risk of sewage leaking into the water supply.

Part 1 of 1 –
Question 10 of 25
4.0 Points
Which of the following statements BEST describes emerging diseases like swine flu?
A.Infectious diseases that were not previously found in humans and typically jump from an animal host to humans.
B.Infectious diseases that existed in the past but for a variety of reasons are increasing in incidence.
C.Infectious diseases that were previously found in humans and typically jump from a human host to animals.
D.Non-infectious diseases that have transformed into infectious diseases.
Part 1 of 1 –
Question 11 of 25
4.0 Points
Which of the following statements about dose-response curves is FALSE?
A.A dose-response curve shows the effect of different doses of a toxicant on a population.
B.Doses lower than the threshold level will have no measurable effect on organisms.
C.The smaller the LD50, the more toxic the chemical.
D.Studies of the dose-response curves of toxicants gives us assurance that for all chemicals there is a threshold level of the chemical so small amounts of chemical pollution pose no health risks
Part 1 of 1 –
Question 12 of 25
4.0 Points
Who wrote Man and Nature, one of the first books discussing human impact on global environmental change?
A.Paul Ehrlich
B.Rachel Carson
C.George Perkins Marsh
D.John Muir
Question 13 of 25
4.0 Points
Chemical A has a toxicity level of 1 and chemical B has a toxicity level of 2. If a mixture of the two chemicals has a toxicity level of 4, what can we say about the results of the mixture?
A.it was additive.
B.it was persistent.
C.it was synergistic.
D.it was antagonistic.
Part 1 of 1 –
Question 14 of 25
4.0 Points
Which of the following criticisms or backlash against the environmental movement in the 20th century are TRUE?
1. In the late 1990s some conservative research groups criticized what they perceived as a biased presentation of environmental issues, particularly the promotion of environmental activism, in schools.
2. Many economists such as Julian Simon have countered the dangers of overpopulation by pointing out that technological advances outpace the negative impacts of population growth.
3. Most of the world’s religions have rejected environmental themes by pointing out that such ideas are contrary to their religious teachings.
a) 1 only
b) 2only
c) 3 only
d) 1 and 2
A.1 and 2
B.2 only
C.1 only
D.3 only
Question 15 of 25
4.0 Points
Which of the following processes plays an important role in the phosphorus cycle?
A.erosion
B.nitrogen fixation
C.combustion
D.cellular respiration
Question 16 of 25
4.0 Points
The nitrogen cycle is one of the most important and complex of the biogeochemical cycles. Why is nitrogen important?
A.It’s an Ozone depleting gas.
B.It’s an essential part of biological molecules such as proteins and nucleic acids (DNA)
C.It’s the most toxic element to living organisms
D.Nitrogen dilutes the oxygen in the atmosphere causing respiratory problems

Part 1 of 1 –
Question 17 of 25
4.0 Points
Herbivores are:
A.primary consumers
B.secondary consumers
C.decomposers
D.scavengers
Part 1 of 1 –
Question 18 of 25
4.0 Points
Which of the following statements about malaria is FALSE:
A.Road clearing in the Amazon has disrupted the natural environment and created habitat for the mosquito larvae to thrive.
B.Mosquitoes do not cause malaria but are the agents of transmission of the disease.
C.It is a non-transmittable disease caused by synthetic chemicals.
D.It is an infectious disease caused by a protozoan parasite.
Part 1 of 1 –
Question 19 of 25
4.0 Points
Which of the following would environmental economists consider natural capital?
A.Water purification by a cattail marsh.
B.A water treatment facility.
C.Increased agriculture made possible by irrigation.
D.A severe hurricane like Katrina.
Part 1 of 1 –
Question 20 of 25
4.0 Points
The soil conservation service was formed after what environmental crisis?
A.soil contamination by toxic waste at love Canal in the 1970s.
B.severe erosion following windstorms in the 1930s.
C.mudslides in California following the San Francisco Earthquake in 1906.
D.severe erosion following forest fires in Yellowstone National Park in the 1980s.
Part 1 of 1 –

Question 21 of 25
4.0 Points
Photosynthesis is:
A.A physical law, which states that when energy is converted from one form to another, some of it is degraded into heat, a less usable form that disperses into the environment.
B.The biological process that captures light energy and transforms it into the chemical energy of organic molecules, which are manufactured from carbon dioxide and water.
C.The measure of the amount of usable energy available to do work in the universe.
D.The process that involves potential energy being converted to kinetic energy.
Part 1 of 1 –
Question 22 of 25
4.0 Points
National income accounts are incomplete estimates of national economic performance because national income accounts do NOT include
A.gross domestic product
B.net domestic product
C.estimates of imported goods and services
D.estimates of external costs such as natural resource depletion and the environmental cost of economic activities
Part 1 of 1 –
Question 23 of 25
4.0 Points
Which of the following is an endocrine disrupter?
A.CFCs
B.mercury
C.ozone
D.carbon dioxide
Part 1 of 1 –
Question 24 of 25
4.0 Points
We measure toxicity by the dose at which adverse effects are produced. Which of the following statements about the dose of and response to toxic agents are true?
A.Lethal doses of particular toxicants for humans are known through doing laboratory tests on human subjects.
B.Doses are expressed in milligrams of toxicant per kilogram of body weight.
C.Sub-lethal doses usually produce acute toxicity whereas lethal doses produce chronic toxicity.
D.Response to a dose depends only on the type of toxicant and not the age or health of the organism.Reset Selection
Part 1 of 1 –
Question 25 of 25
4.0 Points
Why have some plants evolved mutualistic relationships with nitrogen-fixing bacteria?
A. The bacteria utilize nitrogen to produce energy for the plants; the plants provide the source of the nitrogen for the bacteria.
B. The bacteria transform nitrogen into forms useful forms for the plants; the plants provide water and a place for the bacteria to live.
C. The bacteria protect the plant from harmful effects of nitrogen compounds such as ammonia; the plants provide carbohydrates for the bacteria.
D. The bacteria provide natural immunities to the plants; the plants shelter the bacteria from predators.

Environmental Contribution

 
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Chapter 6: DNA, The Molecule of Life

Chapter 6: DNA, The Molecule of Life. Name:       Sec:

 

Chapter 6: DNA, The Molecule of Life

 

Module 6.1. DNA is a polymer of nucleotides.

1. The molecule of heredity is      .

2. Which of the following is used to construct DNA?

A) |_| chromosomes

B) |_| nucleotides

C) |_| double helix

D) |_| carbohydrates

 

3. Create double stranded DNA, by filling in the complementary bases:

A

T

T

G

A

C

4. Match the following terms with their appropriate descriptions: double helix, nucleic acid, nucleotide, backbone.

A) Molecules that contain information to make proteins

B) Consists of a base, five-carbon sugar, and a phosphate

C) Structure of a double-stranded molecule of DNA

D) Identical among all DNA molecules

 

 

5. Complete the following diagram of a nucleotide with the terms: phosphate group, sugar group, base group:

     

 

 

 

 

 

 

 

6. Overall, a molecule of DNA has a negative charge. Which component of DNA gives it this charge?

 

7. A gene is a specific section of DNA that contains the instructions to make a protein. If all molecules of DNA contain a phosphate group, a deoxyribose, and a base, propose an explanation for how the information to make a protein is coded within the DNA molecule.

 

8. If thymine makes up 20% of the bases in a DNA double helix, what percentage of the bases is guanine?

A) |_| 80%

B) |_| 60%

C) |_| 30%

D) |_| 20%

 

 

Module 6.2. During DNA replication, a cell duplicates its chromosomes.

9. If one polynucleotide strand of DNA has ACTTGACTAGCTA as its sequence of bases, what would be the sequence of bases on the opposing strand?

 

10. True or False; If false, make a true statement: DNA must be precisely copied prior to being passed on to the next generation

 

11. The process by which a molecule of DNA is copied is known as      .

 

 

12. Complete the following diagram, which illustrates the nature of DNA replication. Use the following terms: parent double-stranded DNA, daughter double-stranded DNA, template strands, newly synthesized strands. Note some terms may be used more than once.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

13. The following diagram uses colors to illustrate the replication of a chromosome. Use your knowledge of DNA replication to determine whether or not the illustration is accurate. If it is not accurate, briefly explain how to make it correct.

UN_06_004

 

14. Place the following steps of DNA replication in the proper order: DNA fragments are fused together; double helix is pulled apart; new strands of DNA are synthesized.

1.

2.

3.

 

15. A drug that inhibits DNA ligase but not DNA polymerase is added to a cell. Explain how DNA replication would be affected as a result of this drug.

 

Module 6.3. DNA directs the production of proteins via RNA.

16. True or False; if false make a true statement: Nucleic acids store the information to make carbohydrates.

 

17. Two types of nucleic acids that are found in all cells are       and      .

 

18. Which of the following is found in all nucleotides?

A) |_| Phosphate group

B) |_| Ribose

C) |_| Deoxyribose

D) |_| Uracil

 

 

19. Is the following diagram a nucleotide of DNA, RNA, or could it be either one? Briefly explain your answer.

 

20. Complete the following table, which compares and contrasts the structures of DNA and RNA.

  DNA RNA
Unique to…

Common both DNA and RNA

 

21. Certain viruses, like HIV, actually contain RNA. When a virus of this type takes over a host cell, it makes DNA from its RNA. The viral DNA is then used to make an RNA copy, which is used to make viral proteins. In all cells, the flow of genetic information is from DNA RNA proteins. What is the flow of information in viruses (which are not considered cells) like HIV?

 

 

22. You are a biochemist and have been given a test tube containing a small amount of nucleic acid. You have been asked to determine whether it is DNA or RNA. Which of the following would be a characteristic that indicates RNA? Hint: There may be more than one correct answer.

A) |_| Presence of uracil, but no thymine

B) |_| Presence of guanine, but no cytosine

C) |_| Presence of phosphate, but no nitrogen

D) |_| Presence of ribose, but no deoxyribose

 

Module 6.4. Genetic information flows from DNA to RNA to protein

23. True or False; If false, make a true statement: The nucleotide sequence in DNA through the process of information flow comes to produce your characteristics.

 

24. Correct the following: RNA protein DNA

     

 

25. Complete the following diagram, which represents the flow of genetic information in a cell. Use the terms: transcription, translation, DNA, RNA, protein, ribosome, nucleus, cytoplasm, nuclear membrane, nuclear pore. Note that “D” is on the structure behind the arrow, and “E” is on the arrow.

UN_06_007

 

J

I

 

 

G

 

 

 

H

F

E

D

C

B

A

 

 

 

 

 

 

 

 

 

 

 

a.       f.

b.       g.

c.       h.

d.       i.

e.       j.

 

26. Translation converts the information stored in       into      .

A) |_| DNA; RNA

B) |_| RNA; a protein

C) |_| protein; DNA

D) |_| protein; RNA

 

 

27. Briefly explain why you think “mRNA” is a good name for the RNA copy made from DNA.

 

28. Complete the following table, which compares transcription and translation.

  Transcription Translation
Location

Description

 

29. If a strand of DNA has the sequence AAGCTC, transcription will result in which of the following?

A) |_| Single RNA strand with the sequence TTCGAG

B) |_| DNA strand with the sequence TTCGAG

C) |_| Single RNA strand with the sequence UUCGAG

D) |_| DNA strand with the sequence AAGCTC

 

30. A series of three nucleotides that specifies an amino acid is a(n)      .

 

31. A student is given a sequence of DNA as follows: AACTAGCTAGCT, and is asked to provide the mRNA copy that would be made from that sequence during transcription. The student provides the following sequence: TTGATCGATCGA. Is this the correct sequence? If not, explain why it is incorrect and fix it.

 

Module 6.5. Transcription creates a molecule of RNA from a molecule of DNA

 

32.       is the process by which information stored in DNA is copied into mRNA.

 

33. List the following steps of transcription in order: RNA polymerase makes RNA; mRNA leaves the nucleus; RNA polymerase binds the promoter; RNA splicing.

1.

2.

3.

4.

 

34. Complete the following diagram, which illustrates the process of transcription. Use the terms: DNA polymerase, promotor, DNA

UN_06_008

C

B

A

 

 

 

 

 

 

 

 

a. 

b. 

c. 

 

35. The template strand is the DNA strand directly used by the RNA polymerase to make the RNA copy. You are given the following mRNA sequence: AUGCUGAUU. Are you able to determine the sequence of bases on the DNA strand that was not transcribed? Briefly explain your answer either way. (Note: DNA is double stranded. During replication it is unwound and only one strand serves as the template strand. You are being asked if you can determine the sequence of the non-template strand).

 

36. A gene has a sequence of DNA in front of it that directs the RNA polymerase where to begin transcription. This sequence of DNA is the:

A) |_|transcription

B) |_|promoter

C) |_|terminator

D) |_|RNA splicing

 

 

37. You are a graduate student at the University of Iowa and working as a teaching assistant for a freshman introductory biology course. Two of your students have come to you with a question about the sequence of an mRNA transcribed from a gene. The original DNA sequence from which the mRNA was transcribed reads: TGACGATCGTA. The students were asked to provide the sequence of bases in the mRNA and came up with the following mRNA sequence: ACTGCTAGCAT. They are confused because their answer does not match the answer in the book. Briefly explain the very common mistake they made and what the correct sequence would be.

 

38. True or False; If false, make a true statement: The terminator is the sequence of DNA in front of the gene that tells the RNA polymerase where to begin transcription.

 

39. List three ways in which mRNA is modified after transcription:

1.

2.

3.

 

40. Are introns and exons found in DNA or RNA?

 

Module 6.6. Translation involves the coordination of three kinds of RNA.

 

41. True or False; If false, make a true statement: The process of translation occurs within the cytoplasm on a cellular structure known as the mitochondrion.

 

 

42. Which of the following RNA molecules is a component of ribosomes?

A) |_| mRNA

B) |_| rRNA

C) |_| tRNA

D) |_| All of the above

 

43. A set of three nucleotides that specifies an amino acid is called a(n)      .

 

 

44. Which of the following RNA molecules is involved in the process of translation?

A) |_| mRNA

B) |_| rRNA

C) |_| tRNA

D) |_| All of the above

 

45. Complete the following diagram, which illustrates a ribosome:

UN_06_010

 

 

 

 

 

46. Complete the following table, which compares the different kinds of RNA.

  mRNA rRNA tRNA
Function

 

47. Briefly explain why a molecule of tRNA makes a good translator during the process of translation. Be sure to use the terms codon, anticodon, amino acid.

     

 

 

48. According to the table of the genetic code shown here, changing the second base of the codon

A) |_| always changes the amino acid that is encoded.

B) |_| never changes the amino acid that is encoded.

C) |_| sometimes changes the amino acid that is encoded.

D) |_| changes all of the above as noted.

 

49. What codon does every mRNA molecule have in common? What amino acid begins every polypeptide? Use the chart below:

UN_06_011

 

 

50. Match the description to the molecule(s). Each choice should be used only once.

A. DNA

B. mRNA

C. tRNA

D. More than one of the above.

E. None of the above.

A molecule of this will always have an equal percentage of A and G, and an equal percentage of C and T

Has an anticodon and carries an amino acid

Serves as a messenger for taking genetic information from the nucleus to the cytoplasm

Is involved in the process of translation

Is a component of ribosomes

 

51. How many codons are in the following mRNA sequence:

AUGGGGCAGGUAUGA

52. Use the information from question 49 to determine how many amino acids would be in the polypeptide produced from that mRNA.

 

Module 6.7. Translation creates a molecule of protein via the genetic code

 

53. True or False; If false, make a true statement: Ribosomes are part of the cytoplasm.

 

54. Place the following steps of translation in order: elongation, termination, initiation.

1.

2.

3.

 

55. Translation takes place in the ______ on a _________.

A) |_| nucleus; ribosome

B) |_| cytoplasm; ribosome

C) |_| nucleus; mitochondria

D) |_|cytoplasm; lysosome

 

56. A drug that changes the AUG codon to UAC would most likely have what effect on translation? Be brief but specific in your answer.

 

57. If three DNA bases of the template strand are AGT, what is the anticodon of the tRNA that brings the amino acid?

A) |_| UCA

B) |_| TCU

C) |_| AGU

D) |_| TCA

 

58. Complete the following table, which compares the different stages of translation.

  Initiation Elongation Termination
Description

Chapter 6: DNA, The Molecule of Life

 
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Lab 05: Photosynthesis

Lab 05: Photosynthesis. To submit this assignment, students will complete the Lab Worksheet on pages 7-10, then upload their completed document as a DOC or PDF file in Canvas

 

BIO 101 Lab 08: Photosynthesis

 

Notification: If you have a disability that makes it difficult to complete this lab, please contact your instructor. Please provide your instructor a copy of the Memorandum of Accommodation (MOA) from NVCC Disability Support Services.

 

Objectives:________________________________________________________________

· Determine the effects of light on the rate of photosynthesis

· Determine the absorption spectrum of leaf pigments

 

Background:_______________________________________________________________

Sunlight provides the majority of energy for organisms living in most ecosystems, however only a subset of organisms are capable of harvesting this energy. Plants use their chloroplasts to absorb the energy from sunlight. This energy is then stored in the covalent bonds of glucose, a simple sugar, and can be used by the plant for structural purposes (cellulose), as usable energy (ATP generation), or for energy storage (starch). Animals can eat plants to obtain glucose and produce energy through a process called cellular respiration.

 

The overall reaction for photosynthesis is represented by the chemical equation:

 

6 CO2 + 6 H2O + sunlight → C6H12O6 + 6 O2

 

The entire process is complex and involves many enzymatic reactions. You may notice that the photosynthesis reaction is nearly the exact reverse of cellular respiration. From the equation above the three key elements for photosynthesis to occur are carbon dioxide (CO2), water (H2O), and light. If any of the three are missing from the system then photosynthesis will not occur, and glucose production in the plant will be negatively affected.

 

 

 

 

 

 

 

 

 

 

 

There are two phases of photosynthesis: H:BIO101 Lab Manual editsBIO101 XZ VB AS revision 2017Edited AJS512px-Simple_photosynthesis_overview.svg.png

 

1) In the light-dependent phase chlorophyll molecules located in the thylakoid membrane stacks of the chloroplasts absorb the energy from light resulting in the production of ATP and NADPH.

 

2) In the light-independent reactions (the Calvin Cycle) the energy stored in ATP and NADPH is used to ultimately convert carbon dioxide to sugar. The process of taking carbon dioxide from the air to build carbohydrates is called carbon fixation.

 

 

In solution, CO2 can be converted to carbonic acid (H2CO3) when dissolved in water. The carbonic acid will then release hydrogen ions (H+), causing the pH of the solution to decrease.

 

CO2 + H2O H2CO3 HCO3- + H+

 

Bromothymol blue is a pH indicator that changes color based on the pH of a solution. Bromothymol blue turns yellow at lower pH and blue as the pH is increased. This indicator can be used to track respiration (turns yellow as CO2 is added to the system) or photosynthesis (turns blue as CO2 is consumed).

 

Different colors of Bromothymol blue at the indicated pH conditions

 

Light energy is a small part of the electromagnetic spectrum which is visible to the eye. The wavelength of visible light lies between 380 nm and 760 nm. In order to obtain the energy from light, plants must absorb light energy using pigments, namely chlorophyll a, chlorophyll b, carotene and xanthophyll. These pigments show characteristic colors because they do not absorb all light equally. By measuring the absorbance at different wavelengths, the absorption spectrum of the leaf pigments can be obtained.

Materials:__________________________________________________________________

· Internet

 

 

 

Safety:

Follow all standard laboratory safety procedures.

 

Procedure:________________________________________________________________

 

Experiment 1. Observing photosynthesis (work in groups)

1. In the laboratory, you would add about 60 ml of tap water into a beaker and then add about 1 mL of bromothymol blue to the water in the beaker.

 

2. Using a clean straw, you would then gently blow into the solution until the color of the solution turns yellow.

 

3. You would next fill three test tubes 2/3 full with this yellow-colored solution.

 

4. Then, you would place a 3-inch cutting of Elodea into Tube 1 and another 3-inch cutting of Elodea into Tube 2, making sure that the cuttings were completely immersed in the solution. After this, you would wrap Tube 1 with green film. Tube 3 will be left alone and will not contain a plant cutting.

 

5. Watch the following video about setting up the photosynthesis lab from time 0:00 until 5:46.

https://www.youtube.com/watch?v=GWUawtweJGM

 

Ignore the use of a second plant in the video, we only use Elodea in this laboratory activity. In the video, Tubes 1 and 2 contain Elodea plant cuttings and Tube 1 is wrapped in foil instead of green film.

In our photosynthesis lab experiment, we wrap Tube 1 in green film, which only allows green-colored light to reach the Elodea plant cutting. The green film we use ends up having the same effect as the aluminum foil used in the video.

 

6. In Table 1 in the Lab Worksheet, record the color of the solution before blowing into it in the “before exhaling” column.

 

7. In Table 1 in the Lab Worksheet, record the color of the solution after blowing into is in the “after exhaling column.

 

8. Answer question 1 in the Lab Worksheet

 

9. In the laboratory, you would then place the test tubes in front of a light source such as a fluorescent lamp. You would allow the tubes to be exposed to the light for 1 hour.

 

10. Record the color of the solution in each test tube in Table 2 of the Lab Worksheet

 

11. Make a hypothesis about how the color of bromothymol blue solution in each test tube will change and record the color you expect for each tube in Table 2 in the “Expected Color after 1 hour of light exposure” column.

 

When making a hypothesis, consider why the pH-indicating solution turned color in the first place and what might happen if the substance that caused the color change was removed by a plant performing photosynthesis.

 

12. Watch the following video showing the results of photosynthesis lab from time 0:00 until 2:41.

https://www.youtube.com/watch?v=SZsQG_rPJwQ

In the video, Vial A corresponds to Tube 1 in our photosynthesis lab experiment. Ignore Vial B from the video. Vial C in the video corresponds to Tube 2 in our photosynthesis lab experiment and Vial D in the video corresponds to Tube 3 (the control).

Note that in our photosynthesis lab experiment, we wrap Tube 2 in green film, which only allows green-colored light to reach the Elodea plant cutting. The green film we use ends up having the same effect as the aluminum foil used in the video.

 

13. Using the results diagrammed below, fill in Table 2 in the Lab Worksheet

 

 

14. Record the color you observe for each test tube in Table 2 in the “Observed color” column.

 

15. Answer questions 2 – 3 in the Lab Worksheet.

 

 

Experiment 2. Absorption spectrum of leaf extract (work in groups)

1. In the laboratory, you would first turn on the spectrophotometer and allow it to warm up for 15 min.

 

2. You would then insert the blank cuvette (which contains alcohol) in sample holder marked “B” and the cuvette containing leaf extract in sample holder “1”, making sure that the orientation of the cuvettes is correct.

 

3. Then, you would select a wavelength to measure and calibrate the spectrophotometer by measuring the absorbance of the blank cuvette at this wavelength. You would then see the absorbance reading set to 0.

 

4. Now, you would measure the absorbance of the cuvette containing the leaf extract and record the absorbance in Table 3 in the position corresponding to the wavelength you selected.

 

5. You would repeat steps 3 – 4, changing to a different wavelength each time, until all the wavelengths in Table 3 had been measured. Record absorbance readings in Table 3.

 

6. Watch the following video about using a spectrophotometer from time 0:00 until 4:43.

https://www.youtube.com/watch?v=C-M7EtSuD40

Note that the video uses a tube containing a red liquid, while in our photosynthesis lab, we would use a tube containing leaf extract (which would appear green) as our sample tube and a tube containing alcohol (which would be transparent) as our blank tube.

 

7. Table 3 has been partially filled in for you in the Lab Worksheet. Complete Table 3 by filling in the color of light that corresponds to the grouped wavelengths.

 

8. Answer question 4 by making a Line Graph of the data in Table 3.

 

9. Answer questions 5 and 6 based on your Line Graph

 

10. Answer question 7 based on the results of both Experiment 1 and Experiment 2.

 

BIO 101 Lab 08: Photosynthesis Worksheet

 

Name: __________________________ Section: ______________________

 

Data Analysis and Synthesis Questions:

 

Table 1. pH-indicating dye color

Color before exhaling into beaker Color after exhaling into beaker
   

 

1. Why did the solution in the beaker change color after you exhaled?

 

 

 

 

 

Table 2. Observing Photosynthesis

Tube Color before 1 hour of light exposure Expected Color after 1 hour of light exposure Observed color after 1 hour of light exposure
1

(Elodea + green film)

     
2

(Elodea)

     
3

(no plant)

     

 

2. In this experiment , what is the purpose of the tube without the plant?

 

 

 

 

 

 

3. Explain the color change or lack of color change in the three experimental tubes:

 

a. Tube 1:

 

 

 

 

b. Tube 2:

 

 

 

 

c. Tube 3:.

 

 

 

 

Table 3. Absorbance of Different Wavelengths of Light

Color of Light Wavelength (nm) Absorbance   Color of Light Wavelength (nm) Absorbance
  400 0.58     575 0.09
  425 0.82     600 0.12
  450 0.61     625 0.16
  475 0.44     650 0.23
  500 0.22     675 0.53
  525 0.08     700 0.04
  550 0.06     725 0.05

 

 

 

 

4. Make a Line Graph of the absorbance readings versus the wavelength of light data from Table 3. Be sure to include x-axis and y-axis labels and a chart title.

 

 

 

 

 

 

 

 

 

5. Which wavelength(s) and colors of light are most effectively absorbed by leaf pigments (where are the peaks in your Line Graph)?

 

 

 

 

 

 

6. Which wavelength(s) and colors of light are poorly absorbed (where are the valleys in your Line Graph)?

 

 

 

 

 

 

7. Draw a general conclusion about which color(s) of light are best for plant growth based on your data from both the measurement of absorbance of light in leaf extracts and the observations of CO2 use by Elodea cuttings in different light conditions.

 

 

 

 

 

 

 

 

 

 

BIO 101 Lab 08: Photosynthesis 10

Lab 05: Photosynthesis

 
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Biology Experiement Homework2

Biology Experiement Homework2. Transcription and Translation – Introduction

Be sure that you have read over our online lecture this unit on DNA and read pp 177 to 181 in your book before starting. DNA can be a complex concept to grasp, and there is a lot of terminology to keep straight. These first two exercises will focus on transcription and translation, the two processes responsible for taking the information embedded in our DNA and using it to create a protein.

There are segments in our DNA called genes that code for the proteins needed to carry out cellular functions. These genes are a sequence of nucleotides; adenine (A), thymine (T), cytosine (C) and guanine (G) and the specific sequence of these nucleotides is what conveys the information needed to produce a given protein. In humans, the smallest gene is 252 nucleotides long, whereas the largest is more than 2 million nucleotides long! The genetic code is used to decipher the sequence of nucleotides into a sequence of amino acids. The code uses a series of three-nucleotide sequences called codons. Each different codon codes for an amino acid and it is this specific sequence of amino acids that determines what protein is formed.

DNA is found in our nucleus, yet our proteins are synthesized in the cytoplasm. A gene must first be transcribed into a form that can leave the nucleus. Transcription is the process in which a sequence of DNA used to synthesize a complementary strand of messenger RNA (mRNA). This mRNA acts a template and is used to translate the original DNA sequence into a protein, based on the information in its codons and the Genetic Code.

For example, the DNA sequence ATG-CGT-TAG-CGT-ATTC would be transcribed into the mRNA sequence UAC-GCA-AUC-GCA-UAA. Then, using Fig 10.11 on p 180 in your book, you can determine that this mRNA would be translated into the amino acid sequence Tyrosine-Alanine-Isoleucine-Alanine-Stop.

In Exercise 1, you will have the opportunity to demonstrate your understanding of transcription and translation. You will be using the following website; be sure that you are able to access and use the site:

University of Utah. No date. Transcription and Translation

http://learn.genetics.utah.edu/content/molecules/transcribe/ (Links to an external site.)

In Exercise 2, you will apply what you learned in Exercise 1 and evaluate the effect that different types of mutations have on the outcome of transcription and translation. You’ll want to review these mutations on pp 186-187 of your book and in our online lecture on DNA before starting. You will be using the following website; be sure that you are able to access and use the site:

McGraw Hill. No date. Virtual Lab: DNA and Genes

http://www.glencoe.com/sites/common_assets/advanced_placement/mader10e/virtual_labs_2K8/labs/BL_04/index.html  (Links to an external site.)

Finally, in Exercise 3, you will complete a series of calculations to determine the probability of a mutation occurring within a gene that results in a change in protein structure.

UNIT 5 EXPERIMENT ANSWER SHEET Please submit to the UNIT 5 Experiment SUBMISSION LINK no later than Sunday midnight.

SUMMARY OF ACTIVITIES FOR UNIT 1 EXPERIMENT ASSIGNMENT

· Experiment 5 Exercise 1 – Transcription and Translation

· Experiment 5 Exercise 2 – Translation and Mutations

· Experiment 5 Exercise 3 – Mutation Rates

 

Experiment 5 Exercise 1: Transcription and Translation

This exercise will ensure that you have a good understanding of the processes of transcription and translation. To get started, go to the following website:

University of Utah. No date. Transcription and Translation

http://learn.genetics.utah.edu/content/molecules/transcribe/

 

Procedure

A. Read over the information on the first screen and click on the click here to begin to proceed.

B. On the next screen transcribe the give DNA strand.

Table 1. Transcription of the DNA sequence (1.5 pts).

RNA                                            

 

C. Once you have finished transcribing the DNA, you will then translate the RNA sequence. Follow the instructions on the screen.

Table 2. Translation (1.5 pts)

  Codon Amino Acid
Codon 1    
Codon 2    
Codon 3    
Codon 4    
Codon 5    
Codon 6    

 

 

Experiment 5 Exercise 2: Translation and Mutations

Now that you know how to transcribe DNA and translate the mRNA message, let’s take a look at the different types of mutations that might disrupt this process. Review pp 186-187 in your book before beginning. In this exercise you will need to use the following website:

McGraw Hill. No date. Virtual Lab: DNA and Genes http://www.glencoe.com/sites/common_assets/advanced_placement/mader10e/virtual_labs_2K8/labs/BL_04/index.html

Read over the information in the Mutation Guide and close it when you are done. Note that there are several pages; you will need to click on Next to proceed through the Guide. If you want to review this material, you can click on the Mutation Guide button. You are going to run a series of simulations in which an mRNA sequence and its corresponding amino acid sequence is provided. You will be told what type of mutation you will you apply (= Mutation Rule) and you will have to determine the new, mutated mRNA and the resulting protein sequence.

Procedure

A. Click on the Mutate button to get started.

B. Find the Mutation Rule (lower left corner) and enter it into Table 3 below (see the Example provided).

C. Drag the appropriate nucleotides to build the new, Mutated mRNA sequence. If you make a mistake building the new mRNA sequence, drag the correct nucleotide and place it on top of the incorrect one (you cannot actually remove a nucleotide).

D. Once you have generated your Mutated mRNA sequence, you now need to build your Mutated amino acid sequence by matching the appropriate amino acid with each codon. Click on Genetic Code Chart to see the code or you can use Figure 10.11 on p 160 in your book.

NOTE: If you add a STOP codon, do NOT add any more amino acids after it!

 

E. Once you have finished, click on the Check button. If you are correct, then continue with Step F. If you had errors, you will have to Reset the simulation and start over with Step A. Here is what the results look like for the example provided:

F. When you have been successful, enter the Original mRNA sequence and the Original amino acid sequence in the Table below. Then enter the Mutated mRNA and Mutated protein sequence.

G. Click on Reset and repeat Steps A through F four more times so that you end up with FIVE replicates. Do not reuse the same Mutation Rule and do not use the rule used in the example (“the 4th A becomes a C”). If you get the same Mutation rule twice, Reset the simulation and run again.

Do NOT use the same Mutation rule as shown in the example and do NOT use the same Mutation Rule twice!

Table 3. Mutation rules, mRNA sequences and amino acid sequences (10 pts).

Rep Mutation Rule and Sequences
E

X

A

M

P

L

E

Mutation rule: The 4th A becomes a C
  Original mRNA sequence AUG CAC ACG GUG CGA GGG AGU CUG
  Original amino acid sequence Met (Start) – His – Thr – Val – Arg – Gly – Ser – Leu
  Mutated mRNA sequence AUG CAC ACG GUG CGC GGG AGU CUG
  Mutated amino acid sequence Met (Start) – His – Thr – Val – Arg – Gly – Ser – Leu
  Consequence Substitution appears to have had no effect; Arg Arg
1 Mutation rule:
  Original mRNA sequence  
  Original amino acid sequence  
  Mutated mRNA sequence  
  Mutated amino acid sequence  
  Consequence  
2 Mutation rule:
  Original mRNA sequence  
  Original amino acid sequence  
  Mutated mRNA sequence  
  Mutated amino acid sequence  
  Consequence  
3 Mutation rule:
  Original mRNA sequence  
  Original amino acid sequence  
  Mutated mRNA sequence  
  Mutated amino acid sequence  
  Consequence  
4 Mutation rule:
  Original mRNA sequence  
  Original amino acid sequence  
  Mutated mRNA sequence  
  Mutated amino acid sequence  
  Consequence  
5 Mutation rule:
  Original mRNA sequence  
  Original amino acid sequence  
  Mutated mRNA sequence  
  Mutated amino acid sequence  
  Consequence  

 

Questions

1. What is a silent mutation? Did you see any examples of this in your mutations above? If so, which mutation rule(s) generated it? Cite your sources (2 pts).

 

2. What is a missense mutation and how does it differ from a nonsense mutation? Did you see examples of either of these types of mutation and if so, which mutation rule(s) generated it? Cite your sources (2 pts).

3. What is a frame-shift mutation and why are they so damaging? Did you see any examples of this in your mutations above? If so, which mutation rule(s) generated it? Cite your sources (2 pts).

 

4. Find a genetic disorder that develops as a result of one of the types of genetic mutations we have examined in this exercise. Identify the disorder and briefly describe the mutation responsible. Cite your sources (3 pts).

 

 

Experiment 5 Exercise 3: Mutation Rates

We learned in our second exercise that not all mutations have an observable effect. Yet the risk of a mutation being damaging is fairly significant, so it is important to understand the probability of them occurring. In this exercise, we are going to calculate the probability of a mutational event within a gene. You are given the necessary information below to complete the calculations. Do not let them overwhelm you; this is simple math, so think things through.

Assume that:

· there are approximately 3,000,000,000 base pairs in the mammalian genome (genes constitute only a small portion of this total)

· there are approximately 10,000 genes in the mammalian genome

· a single gene averages about 10,000 base pairs in size

 

Questions

1. Based on the assumptions above, in the mammalian genome, how many total base pairs are in all the mammalian genes? Show your math (2 pts).

2. What percentage (%) of the total genome does this represent? Show your math (2 pts).

 

3. What is the chance (%) that a random mutation will occur in any given gene? Show your math (2 pts).

 

4. Only 1 out of 3 mutations that occur in a gene result in a change to the protein structure. What is the probability that a random mutation will change the structure of a protein? Show your math (2 pts).

 

UNIT 1 Experiment Grading Rubric

Component Expectation Points
Experiment 5 Exercise 1 Demonstrates an understanding of the process of transcription and translation (Table 1 and 2). 3 pts
Experiment 5 Exercise 2 Correctly implements the proper mutation and transcribes the mRNA correctly (Table 3). 10 pts
  Demonstrates an understanding of the different types of mutations and their consequences (Questions 1-4). 9 pts
Experiment 5 Exercise 3 Correctly calculates the necessary information (Questions 1-4). 8 pts
TOTAL   30 pts

 

 

 

1. Explain the four roles that DNA plays in cells? How are these roles influenced by DNA’s structure? Be sure you demonstrate your understanding of DNA’s structure in your answer.

 

Citation(s):

 

2. Match the terms with the most suitable description.     _____ genetic code             a.  Examples of RNA processing     _____ promoter                   b. Sequence of three nucleotides that code for an amino acid.     _____ exon                          c. Location on DNA where RNA polymerase attaches.     _____ intron                        d. Sequence of three nucleotides that is complementary to a codon triplet.     _____ anticodon                  e. Portion of a gene that is excised from the RNA transcript.      _____ codon                        f.  Rules that convert a nucleotide sequence into a protein.     _____ cap and tail               g. Parts of a gene that are expressed.

3. Briefly explain the differences among messenger RNA, transfer RNA and ribosomal RNA in terms of the roles they play in transcription and translation and where they are found in the cell.

 

Citation(s):

 

4. Using the genetic code table (Fig 10.11 on p 180), take the following DNA sequence and complete the following. Note that the mRNA is generated from the complementary DNA strand.                                           T A C C C C A T G T A A C A T A C C A C T

Complementary DNA strand _______________________________________________

mRNA strand ___________________________________________________________

Amino acid sequence _____________________________________________________

 

5. Part of the coding sequence of a gene produces an mRNA sequence of  A U G A A G G C U C C U C C A A G C G G C

What is the DNA sequence __________________________________________________

What is the amino acid sequence _____________________________________________

6. Review pp 178-185 in your book and view the following animation. Then complete the following table. You may need to watch it the video more than once to catch the details.

Genome British Columbia. Gene Expression. 2007. Web. 25 July 2016. https://www.youtube.com/watch?v=OEWOZS_JTgk (Links to an external site.) undefined

Question Transcription Translation
What is it, in brief?    
Where does it occur in the cell?    
What is the product?    
Describe how the product is modified to reach its final form.    

Citation(s):

 

Updated April 2015

Biology Experiement Homework2

 
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