Hanging-drop and wet-mount preparations

Hanging-drop and wet-mount preparations. Week 1 – Review Sheet

Exercise 1: Hanging-drop and wet-mount preparations

1. How does true motility differ from Brownian movement?

2. What morphological structure is responsible for bacterial motility?

3. Why is a wet preparation discarded in disinfectant solution or biohazard container?

4. What is the value of a hanging-drop preparation?

5. What is the value of a wet-mount preparation?

Exercise 2: Simple stains

1. Define acidic and basic dyes. What is the purpose of each?

2. What is the purpose of fixing a slide that is to be stained?

3. Why are the specimens to be stained suspended in sterile saline or distilled water?

4. How does a stained preparation compare with a hanging drop for studying the morphology

and motility of bacteria?

5. List at least three types of bacteria whose names reflect their shapes and arrangements,

and state the meaning of each name.

Exercise 3: Gram stain

1. What is the function of the iodine solution in the Gram stain? If it were omitted, how would

staining results be affected?

2. What is the purpose of the alcohol solution in the Gram stain?

3. What counterstain is used? Why is it necessary? Could colors other than red be used?

4. What is the advantage of the Gram stain over a simple stain such as methylene blue?

5. In what kind of clinical situation would a direct smear report from the laboratory be of

urgent importance?

Hanging-drop and wet-mount preparations

 
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Properties Of Water Lab

Properties Of Water Lab. Name_________________ per____ date______ mailbox______

Properties of Water Lab

Water’s chemical formula is H2O. As the diagram to the left shows, that is one atom of oxygen bonded to two atoms of hydrogen. The hydrogen atoms are “attached” to one side of the oxygen atom, resulting in a water molecule having a positive charge on the side where the hydrogens reside and a negative charge on the other side, where the oxygen atom resides. This separation of charge on opposite ends of the molecule is called polarity. Since opposite

image1.png

electrical charges are attracted to one another, water molecules tend to be attracted to each other. This makes water tend to be

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kind of “sticky.” As the right-side diagram shows; the hydrogen end of the bottom water molecule’s (positive charge) is attracted to the oxygen end of the above water molecule’s (negative charge) and so on and so forth up the chain. This property of water is known as cohesion. All these water molecules attracting each other mean they tend to clump together. This is why water drops are, in fact drops! If it wasn’t for gravity, these drops would be ball shaped or spherical as is the case aboard the International Space Station.

Even if it doesn’t form a perfect sphere on Earth, we ought to be happy water has the properties it does. Indeed without these properties we could not exist. For instance, for many of the same reasons water is “sticky” it is also known as the “Universal Solvent” because it dissolves perhaps more substances than any other liquid on Earth. This means that wherever water goes, either through the ground, our bodies, the sky or oceans it carries with it valuable chemicals, like minerals and nutrients.

Ever wonder, how a water strider walks across the surface of a pond without sinking? The surface water bears a remarkable property. On the molecular scale, it acts like a stretched elastic membrane or “skin.” This property is referred to as surface tension and is largely due to cohesion between adjacent water molecules. It is caused by the attraction of the particles in the surface layer and the bulking up of the liquid below, tending to minimize surface area as molecules strive for the optimal pole to pole bonding arrangement. The whole effect causes tightness at the surface, which resists the step of a water strider’s foot, supports a leaf temporarily and causes water to bead up on windows, certain clothing or a duck’s back. It can slow the wetting process of certain fabrics and inhibit the cleaning process too.

Surface tension can however be reduced. Increase the water’s temperature and kinetic energy starts to move molecules about again, while also promoting evaporation. The water vapor now above can linger in transition grabbing at remaining water molecules along the surface boundary and tugging at them from above. Bring water to a boil and surface tension is all but lost.

The addition of substances such as soap or detergent (known as surfactants) can reduce surface tension by increasing the spreading and wetting properties of water. Surfactant molecules look like tadpoles. The head is hydrophilic meaning attracted to water and typically soluble. Meanwhile, the tail of

a surfactant is composed of fatty material that is insoluble in water and allows grease, oils or other fats to stick to it. This property of not dissolving in water makes it hydrophobic

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1

meaning to repel water. Surfactant molecules break cohesion and surface tension allowing water to contact more dirt particles, making fabrics wetter and allowing materials to be cleaned.

Water striders can walk on the surface of water despite having a density themselves greater than that of water. Meaning they ought to sink if not for water’s properties. However, surface tension and tiny hairs on their feet which are hydrophobic allow them to keep from getting wet and keeps them afloat.

1. Fill in the properties of water we investigate during this lab, from the reading above.

a. _____________ relating to the separation of charges in a molecule

b. _____________ meaning to repel water, literally “fear of water”

c. _____________ term for a chemical which can break surface tension

d. _____________ this term describes the force of attraction between adjacent water molecules.

e. _____________ meaning attracted to water or literally “water loving”

2. ______What is the property of water that describes a water molecule’s charge ?

a. polarity b. surface tension

c. cohesion

 

d. surfactant

3. ______What is the property of water that describes the force of attraction between water molecules?

a. polarity b. hydrophobic

c. cohesion

 

d. surfactant

4. ______What is the property of water allows a water strider to walk on the surface of water?

a. polarity b. surface tension

c. cohesion

 

d. adhesion

5. Definitions: use the reading.

Define: polarity- ________________________________________________________________

_____________________________________________________________________________

_____________________________________________________________________________

Define: cohesion- ________________________________________________________________

_____________________________________________________________________________

_____________________________________________________________________________

Define: surface tension-___________________________________________________________

_____________________________________________________________________________

_____________________________________________________________________________

2

Water QUESTIONS

1. Explain why water is the universal solvent using a complete sentence. Use the word solubility or dissolve.

_____________________________________________________________________________

_____________________________________________________________________________

_____________________________________________________________________________

2. What is a polar molecule? Explain. Use the words positive, negative and charge.

_____________________________________________________________________________

_____________________________________________________________________________

_____________________________________________________________________________

3. Which end of the water molecule is negative? Which end is positive?

_____________________________________________________________________________

_____________________________________________________________________________

image4.jpg image5.jpg

Fill in the blank:

Surface tension causes water to ______________________ on surface like glass.

Water molecules are _____________________ to other water by a process called cohesion.

Surfactants are used to break cohesion between water molecules, making surfaces wetter and allowing water to _____________ away dirt.

 

STATION 1: PENNY

1. Place a penny flat on the table.

2. Hypothesize – how many drops of water do you think the penny could hold before water spills over the edge? Experiment with the head side verses the tails side of the coins.

3. Using a dropper, count how many drops the penny holds until it spills over. Draw how the water appeared on the penny right before it spilled off.

 

Hypothesis – Estimated # of drops  Heads ________ Tails ________

 

Record the actual # of drops ________

Properties Of Water Lab

 
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Different biotechnology techniques.

Different biotechnology techniques.. You will be watching some short films on using gene therapy to treat conditions in somatic cells.  You will submit your responses to the questions on BB as either a Word Doc or PDF file.

Something different.  Watch the two short videos about different biotechnology techniques.

https://youtu.be/ytz2Dn9qpDU

https://youtu.be/GGEiaDau7hU

Both of these discuss using gene therapy.  This technology has many benefits, however it is still very young.  As described in one of my lectures there are two types of gene therapy.  We hit on editing the gametes when we did our discussion post on CRISPR.  The type of therapy described in these videos goes over somatic gene therapy.

For this assignment you will give your opinion on somatic gene therapy.  In one to two paragraphs state your views on somatic gene therapy.  If you were personally affected with a condition that could be treated with gene therapy would you enroll in a clinical trial to try it knowing that it may not work and may have potential side effects?  What if it was your child that had a condition that could be treated with gene therapy and was offered a spot in a similar clinical trial?  If the therapy was only tested in model organisms, would you wait to see it tested in humans first?  If there another option like the use of human stem cells to test this therapy on before human trials?   You can justify your opinion on topics like severity of the disease, types of symptoms, age, percent success rate, and so on.  You are not limited to only talking about gene therapy used in eyes like in these videos.  You can research up on current gene therapies being offered too.  I am not picky on style, margins, font and so on.  You just need to justify your opinions on the topic.  Lack of reasoning will lead to deductions on your grade.  Remember that this is an opinion paper and I cannot count your opinion wrong.

Different biotechnology techniques.

 
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Ecological Footprint Analysis of Tropical Rainforest Deforestation

Ecological Footprint Analysis of Tropical Rainforest Deforestation. 13 Ecological Footprint Analysis of Tropical Rainforest Deforestation

Use the table below to answer the questions that follow.

Country Area of

tropical rain forest (square kilometers)

Area of

deforestation per year (square kilometers)

Annual rate of tropical rainforest loss
A 1,800,000 50,000 .03%
B 55,000 3,000  
C 22,000 6,000  
D 530,000 12,000  
E 80,000 700  
       

 

1.  What is the annual rate of tropical rain forest loss, as a percentage of total forest area, in each of the five countries?  Answer by filling in the blank column in the table.

2.  What is the annual rate of tropical deforestation collectively in all of the countries represented in the table?

 

3.  According to the table, and assuming the rates of deforestation remain constant, which country’s tropical rain forest will be completely destroyed first?

 

4.  Assuming the rate of deforestation in country C remains constant, how many years will it take for all of its tropical rain forests to be destroyed?

 

5.  Assuming that a hectare (1.0 hectare = 0.01 square kilometer) of tropical rain forest absorbs 0.85 metric tons (1 metric ton = 2,200 pounds) of carbon dioxide per year, what would be the total annual growth in the carbon footprint (carbon emitted but not absorbed by vegetation because of deforestation) in metric tons of carbon dioxide per year for each of the five countries in the table?

A =

B =

C =

D =

 

E =

Ecological Footprint Analysis of Tropical Rainforest Deforestation

 
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